Knowledge Now in Experimental Biology: Working with Proteins is a new six-part laboratory video series that fills a gap in the tools available for teaching students the fundamentals of working with proteins. Through a lively mixture of lab bench demonstrations, vivid scientific animations, and interviews with researchers, this series offers a memorable introduction to many of the most important tools in the modern research biologist's toolkit.The first video in this series covers basic principles and demonstrates laboratory techniques for lysing a cell, separating cellular components by preparative centrifugation, and purifying and concentrating a fraction containing a protein of interest by ammonium sulfate precipitation.Video Two details three of the most common chromatographic approaches used to isolate a protein of interest from a crude cell extract: Gel filtration chromatography, ion exchange chromatography, and affinity chromatography. Video Three describes how SDS-polyacrylamide gel electrophoresis is used to estimate the monomer molecular weights by measuring the migration of denatured molecules in an electric field.The fourth video lays out a strategy for designing a good activity assay, emphasizing criteria that must be met to yield valid results. Video Five examines the wide range of techniques now available for analyzing protein primary structure.The final video focuses on how researchers determine the three-dimensional structure of proteins.
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