This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture.
Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering. These and other developments have been incorporated into this rigorously revised and expanded sixth edition of Culture of Animal Cells, the leading text in the basic methodology of cell culture.
This authoritative volume offers a complete background related to growth of animal cells in culture. It contains many specialized protocols and advice on how to apply some of the basic protocols to teaching cell culture. Beginning with laboratory design, safety, validation, and bioethics, then continuing with preparation of media, primary culture, and cell lines through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the unparalleled coverage includes:
An all-new section addressing the additional caution required once a primary culture is converted to a cell line
Differentiation in stem cells and reprogramming of adult cells to form stem cells
A new section and protocol on cryopreservation by vitrification
Separate sections dealing with epithelial cells, mesenchymal cells, neurectodermal cells, and hematopoietic cells, a new chapter on germs cells/stemcells/amniocytes
A new section on organotypic culture
Increased emphasis on contamination and misidentification (including cross-contamination) with revised protocols for detection and elimination
A problem-solving chapter, glossary of terms, advice on sourcing materials, and list of suppliers
This landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells and is indispensable for those in the field, including clinical and biopharmaceutical researchers and scientists, graduate students, laboratory managers in cell and molecular biology labs and genetics labs, trainees, and technicians.
Q&A with Author R. Ian Freshney, PhD.
|Author R. Ian Freshney, PhD.|
"synopsis" may belong to another edition of this title.
R. IAN FRESHNEY, PhD, is an honorary Senior Research Fellow in the Centre for Oncology and Applied Pharmacology, part of the Cancer Research UK Beatson Laboratories at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique.
"About this title" may belong to another edition of this title.
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Book Description Wiley-Blackwell, 2010. Book Condition: New. Brand New, Unread Copy in Perfect Condition. A+ Customer Service! Summary: This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer. Bookseller Inventory # ABE_book_new_0470528125
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Book Description John Wiley and Sons Ltd, United Kingdom, 2010. Hardback. Book Condition: New. 6th Revised edition. 282 x 218 mm. Language: English Brand New Book. This is the sixth edition of the leading text in the basicmethodology of cell culture, worldwide. Rigorously revised, itfeatures updates on specialized techniques in stem cell researchand tissue engineering; updates on molecular hybridization, somaticcell fusion, hybridomas, and DNA transfer; new sections onvitrification and Organotypic Culture, and new chapters onepithelial, mesenchymal, neurectodermal, and hematopoietic cells;germs cells/stemcells/amniocytes; and non-mammalian/avian cells. Itis written for graduate students, research and clinical scientists,and technicians and laboratory managers in cell and molecularbiology labs and genetics labs. PowerPoint slides of the figures as well as other supplementarymaterials are available at a companion website: Bookseller Inventory # AAH9780470528129