YAC Libraries: A User's Guide (University of Wisconsin Biotechnology Center Biotechnical Resource Series)
ISBN 13: 9780716770145
YAC Libraries: A User's Guide (University of Wisconsin Biotechnology Center Biotechnical Resource Series)
An indispensable guide to an emerging technology, this unique book introduces detailed methods for the construction, screening, and use of DNA sequences cloned into yeast artificial chromosome (YAC) vectors and the applications of YACs to gene hunting. Also covered are the reconstruction of large genes and the analysis of YACs by insertion back into mammalian cells--a method that may lead to the therapeutic use of YAC clones. The methods described are drawn from work on human DNA, but are universal to yeast artificial chromosomes used in other organisms. YAC Libraries is the third volume in the UWBC Biotechnical Resource Series with Dr. Richard Burgess, Director of the University of Wisconsin Biotechnology center, as Series Editor. The series is dedicated to providing practical guidance to researchers exploring new technologies and services.
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About the Author:
David L. Nelson, Institute for Molecular Genetics, Baylor College of Medicine. Bernard Brownstein, Center for Genetics in Medicine, Washington University School of Medicine.
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