Molecular Microbiology Techniques Part A

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9780120443055: Molecular Microbiology Techniques Part A

The new series METHODS IN MOLECULAR GENETICS provides practical experimental procedures for use in the laboratory. Because the introduction of molecular genetic techniques has revolutionized biological research, a wide range of methods is covered. This volume of METHODS IN MOLECULAR GENETICS presents up-to-date practical molecular biology and genetics techniques of the analysis of microbial genes and chromosomes, including those od eukaryotic andf prokaryotic cells and plasmids.


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* Methods presented for easy use and ready adaptation to new systems

* Detailed experimental protocols included for:
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* Eukaryotic microbes - yeast (mutants, transposons, viruses), parasites (gene identificationa dn regulatino), slime mold (transformation)
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* Bacterial DNA and Chromosmes - codon usage, quantitation of RNA transcription, challenge phage, cell division, motility and chemotaxis
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* Bacterial Gene Analysis - gel shift assay, DNase 1 footprinting, gene fusions, membrane protein genes, oxidative stress genes
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* Plasmids - assays for DNA and DNA-binding proteins

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