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This volume presents a compilation of experimental methods and principles, with detailed protocols and examples. Techniques which have demonstrated value in problem-solving are included.
Intended for biochemists, cell biologists, microbiologists, pharmacologists, biomedical scientists, biophysicists, analytical chemists, and physiologists, this book focuses on mass spectrometry. Topics discussed include the methods of ionization, liquid chromatography, continuous flow fast atom bombardment mass spectrometry, collision-induced dissociation, enzymatic and chemical digestion of proteins, and constituents of nucleic acids. Other titles in this series include Guide to Yeast Genetics and Molecular Biology and Retinoids, Part B: Cell Differentiation and Clinical Applications.
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Praise for the Volume
"This volume will be a most welcome addition to the shelves of those working with mass spectrometry (MS) applications... The book is useful and comprehensive, offering a concise treatment of experimental principles along with detailed descriptions of specific methods... This book is a gold mine of information. The standards of excellence established in the Methods in Enzymology series have been continued in this volume. The 70 authors and the editor are to be congratulated."
"The Methods in Enzymology series has a well-established reputation for producing carefully edited, authoritative, practical summaries of current research by experts in various specialty fields. J.A. McCloskey has continued that high standard in his selection of 70 scientists who have written 47 chapters... occupies a niche not filled previously by any single publication in the field... There are six very useful appendices to help guarantee that this volume will find a permanent home close to the operator of mass spectrometer (elemental mass and abundance data, ions in 11 defined reference materials for different types of ionization, characteristic spectra of seven FAB matrices, calculation of isotopic abundance distributions, nomenclature for peptide fragment ions, and residue masses for peptides). In summary, this book is an extremely valuable resource for the practicing mass spectroscopist. Due to the inclusion of general introductory material, it would be an excellent text for a course in biological mass spectrometry directed toward graduate students in chemistry or biochemistry."
Praise for the Series
"The Methods in Enzymology series represents the gold-standard."
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
--CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
--AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
"A series that has established itself as a definitive reference for biochemists."
--JOURNAL OF CHROMATOGRAPHY
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Book Description Academic Press. Hardcover. Condition: New. 0121820947. Seller Inventory # NJ001430
Book Description Academic Press, 1991. Condition: New. book. Seller Inventory # M0121820947