This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
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Praise for the Series "The Methods in Enzymology series represents the gold-standard." ( Neuroscience)
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"...including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." (Bio/Technology)
"...It should be on the shelves of all libraries in the world as a whole collection." (Chemistry in Industry)
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation..." (American Society of Microbiology News)
"If we had some way to find the work most often consulted in the laboratory, it could well be...Methods in Enzymology." (Enzymologia)
"A series that has established itself as a definitive reference for biochemists." (Journal of Chromatography)About the Author:
Daniel Lee Purich has been at the forefront of biochemistry research for more than 25 years. He was awarded the National Institutes of Health Research Career Development Award from 1977-1982, the Plous Teaching Award (the University of California Santa Barbara Campus-Wide Teaching Award) in 1977, has been a member of the National Institutes of Health Biochemistry Study Section from 1982-1985, and a member of the Journal of Biological Chemistry Editorial Board from 1981-1986. He has been a member of the American Society of Biological Chemists, the American Chemical Society, the New York Academy of Sciences, the Biochemical Society, and the American Society for Cell Biology. Dr. Purich is currently a Professor and Chairman of the Department of Biochemistry and Molecular Biology at the Florida College of Medicine. He is the author and editor of numerous scientific publications.
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