The Zebrafish: Genetics and Genomics, Volume 60

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9780122121722: The Zebrafish: Genetics and Genomics, Volume 60

This is the second volume of a two-volume, comprehensive treatment of the methodologies used in researching the Zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications.

Key Features
* Details state-of-the-art zebrafish protocols in a single-source reference
* Presents methods and reagents in user-friendly format
* Delineates critical steps and pitfalls of he procedures
* Illustrates techniques with full-color plates
* Summarizes many new and interesting developmental mutants
* Includes appendices with strain information and a compendium of zebrafish World Wide Web sites
* Is relevant to clinicians interested in vertebrate models of human congenital diseases

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From the Back Cover:

The Zebrafish: Genetics and Genomics presents laboratory protocols and reviews that focus on Danio rerio, the tropical, freshwater fish that has emerged as an extremely powerful molecular-genetic model system for analysis of vertebrate development. This volume describes strategies and methods for genetic screening to obtain developmental mutants and techniques for mapping mutations and cloning the causative genes. Accessible to novices and "old hands" alike, this volume provides a comprehensive reference to current genetic and genomic approaches in zebrafish.
Also available is the companion volume, Zebrafish: Biology, which describes the embryology of the zebrafish, presents methods for cell culture and for the study of gene expression and function in development, highlights cellular techniques for analysis of morphogenesis, and concludes with protocols for the study of, and descriptions of mutations that perturb organogenesis.
Key Features
* Details state-of-the-art zebrafish protocols in a single-source reference
* Presents methods and reagents in user-friendly format
* Delineates critical steps and pitfalls of he procedures
* Illustrates techniques with full-color plates
* Summarizes many new and interesting developmental mutants
* Includes appendices with strain information and a compendium of zebrafish World Wide Web sites
* Is relevant to clinicians interested in vertebrate models of human congenital diseases

About the Author:

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

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