GUS Protocols: Using the GUS Gene as a Reporter of Gene Expression

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9780122740107: GUS Protocols: Using the GUS Gene as a Reporter of Gene Expression

The gb-glucuronidase (GUS) gene is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes are useless. Thus, the GUS gene is the reporter gene of choice for transgenic plant research. Not only can this assay be used to detect whether a gene is being expressed, but it can be used to determine the location of the gene product within cells. Low cost is another advantage of this assay.
GUS Protocols provides instructions and essential background information that will enable researchers to effectively use the GUS gene as a reporter of the expression of introduced genes.

Key Features
* First book on the GUS reporter system
* Up-to-date reference lists following each of the fourteen chapters
* Comb-bound for convenient bench-top use
* Written by leading authorities including R.A. Jefferson, inventor of the GUS assay
* Illustrated with color
* Easy-to-follow procedures for
* Using the GUS system in both plants and animals
* GUS detection by fluorometric, spectrophotometric, and histochemical methods
* Fast, automated assays
Background information on
* The gb-glucuronidase reaction
* Substrates
* Instrumentation
* Construction of GUS fusion vectors

"synopsis" may belong to another edition of this title.

From the Back Cover:

The b-glucuronidase (GUS) gene is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes are useless. Not only can this assay be used to detect whether a gene is being expressed, but it can be used to determine the location of the gene product within cells.
GUS Protocols provides instructions and essential background information that will enable researchers to effectively use the GUS gene as a reporter of the expression of introduced genes.
Key Features and Benefits
* Written by leading authorities
* Foreword by R.A. Jefferson, inventor of the GUS assay
* Up-to-date reference lists following each of the fourteen chapters
* Illustrated with nineteen full-color photographs
* Offers clearly written, straightforward, easy-to-follow procedures for
* Using the GUS system in both plants and animals
* GUS detection by fluorometric, spectrophotometric, and histochemical methods
* Fast, automated assays
* Provides background information on
* The b-glucuronidase reaction
* Substrates
* Instrumentation
* Construction of GUS fusion vectors

Excerpt. © Reprinted by permission. All rights reserved.:

The first book on the GUS reporter system.

"About this title" may belong to another edition of this title.

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Sean R. Gallagher (Editor)
Published by Academic Press (1992)
ISBN 10: 0122740106 ISBN 13: 9780122740107
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Published by Academic Press (1992)
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Book Description Academic Press, 1992. Plastic Comb. Book Condition: Brand New. spiral-bound edition. 221 pages. 9.00x6.25x0.50 inches. In Stock. Bookseller Inventory # zk0122740106

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Published by Academic Press (1992)
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