Guide to Yeast Genetics and Molecular Biology, Volume 194: Volume 194: Guide to Yeast Genetics and Molecular Biology (Methods in Enzymology, Vol.194)

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9780123106704: Guide to Yeast Genetics and Molecular Biology, Volume 194: Volume 194: Guide to Yeast Genetics and Molecular Biology (Methods in Enzymology, Vol.194)

Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast.

Basic Methods in Yeast Genetics**Physical and genetic mapping**Making and recovering mutants**Cloning and Recombinant DNA Methods**High-efficiency transformation**Preparation of yeast artificial chromosome vectors**Basic Methods of Cell Biology**Immunomicroscopy**Protein targeting assays**Biochemistry of Gene Expression**Vectors for regulated expression**Isolation of labeled and unlabeled DNA, RNA, and protein

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Edited by Christine Guthrie, Gerald R. Fink, John N. Abelson and Melvin I. Simon

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"This book will be a valuable resource both for beginners and for current practitioners. It should enable newcomers to set up a yeast laboratory and master basic manipulations."
--CAMBRIDGE SCIENTIFIC ABSTRACTS
"Because this book covers so much in what is a technique-rich area, no researcher in the field will want to be without it... This is an exceptional book. While it is indeed a guide to many techniques and methods, in the final analysis, it is more than that. It is also a proclamation that yeast biology is now in the main stream."
--ASM NEWS

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John N. Abelson (Editor), Melvin I. Simon (Editor), Christine Guthrie (Editor), Gerald R. Fink (Editor)
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Editor-John N. Abelson; Editor-Melvin I. Simon; Editor-Christine Guthrie; Editor-Gerald R. Fink
Published by Academic Press (1991)
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