Guide to Yeast Genetics and Molecular Biology, Volume 194: Volume 194: Guide to Yeast Genetics and Molecular Biology (Methods in Enzymology, Vol.194)

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9780123106704: Guide to Yeast Genetics and Molecular Biology, Volume 194: Volume 194: Guide to Yeast Genetics and Molecular Biology (Methods in Enzymology, Vol.194)

Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast.

Basic Methods in Yeast Genetics**Physical and genetic mapping**Making and recovering mutants**Cloning and Recombinant DNA Methods**High-efficiency transformation**Preparation of yeast artificial chromosome vectors**Basic Methods of Cell Biology**Immunomicroscopy**Protein targeting assays**Biochemistry of Gene Expression**Vectors for regulated expression**Isolation of labeled and unlabeled DNA, RNA, and protein

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Edited by Christine Guthrie, Gerald R. Fink, John N. Abelson and Melvin I. Simon

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Editor-John N. Abelson; Editor-Melvin I. Simon; Editor-Christine Guthrie; Editor-Gerald R. Fink
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