Low-Abundance Proteome Discovery: State of the Art and Protocols - Softcover

Boschetti, Egisto; Righetti, Pier Giorgio

Softcover

ISBN 10: 0124017347 ISBN 13: 9780124017344

Publisher: Elsevier, 2013

Synopsis

Low-Abundance Proteome Discovery addresses the most critical challenge in biomarker discovery and progress: the identification of low-abundance proteins. The book describes an original strategy developed by the authors that permits the detection of protein species typically found in very low abundance and that may yield valuable clues to future discoveries. Known as combinatorial peptide ligand libraries, these new methodologies are one of the hottest topics related to the study of proteomics and have applications in medical diagnostics, food quality, and plant analysis. The book is written for university and industry scientists starting proteomic studies of complex matrices (e.g., biological fluids, biopsies, recalcitrant plant tissues, foodstuff, and beverage analysis), researchers doing wet chemistry, and graduate-level students in the areas of analytical and biochemistry, biology, and genetics.

• Covers methodologies for enhancing the visibility of low-abundance proteins which, until now, has been the biggest challenge in biomarker progress
• Includes detailed protocols that address real-life needs in laboratory practice
• Addresses all applications, including human disease, food and beverage safety, and the discovery of new proteins/peptides of importance in nutraceutics
• Compiles the research and analytic protocols of the two scientists who are credited with the discovery of these landmark methodologies, also known as combinatorial peptide ligand libraries, for the identification of low-abundance proteins

About this title

From the Back Cover:

Low-Abundance Proteome Discovery: State of the Art and Protocols addresses the biggest challenge in the field of protein biomarkers–the detection of low-abundance proteomes–by using an original strategy developed by the authors. This strategy, known as combinatorial peptide ligand libraries, permits the detection of species of proteins that are typically present in very low abundance and that may yield valuable clues to future discoveries. Low-Abundance Proteome Discovery: State of the Art and Protocols first presents an overview of low-abundance proteins and their importance for research and diagnostics, followed by a survey of conventional prefractionation methods in proteome analysis. Then, it describes the principles and applications of peptide ligand libraries as a unique approach for the detection of low-abundance proteins. Finally, it offers a detailed description of laboratory practices and recipes that will help you succeed in detecting and identifying low-abundance proteins in various situations.

Key features:

• Covers novel methodologies for enhancing the visibility of low-abundance proteins
• Includes detailed protocols for the detection, identification, and quantification of low-abundance proteins that address real-life needs in laboratory practice
• Addresses all applications, including human disease, food and beverage safety, and the discovery of new proteins/peptides of importance in nutraceuticals

Review:

"The book was written by the inventors of and leading experts in the technology and provides a comprehensive overview of what has been achieved with this relatively new approach. The detailed description of important experimental parameters and the protocols provided in this book make it a unique resource for all practitioners in the field and all scientists interested in the technology."--Analytical & Bioanalytical Chemistry, February 2014 "Boschetti and Righetti introduce the new technique of combinatorial peptide ligand libraries for identifying low-abundance peptide fragments. The standard proteomics techniques of mass spectrometry, 2D electrophoresis and chromatography are deliberately given brief treatment only as prefractionation tools in favor of new methodologies."--Reference & Research Book News, October 2013

About this edition

• Publisher: Elsevier
• Publication date: 2013
• ISBN 10: 0124017347
• ISBN 13: 9780124017344
• Binding: Paperback
• Edition number: 1
• Number of pages: 364