Tetrahymena thermophila is emerging as a powerful experimental system for functional genetics. The ciliated protozoan offers numerous advantages, not the least of which is the ability to eliminate any specific gene of interest and then to evaluate the effect of that mutation on the living cell. Past investigations with T. thermophila have yielded several key discoveries, including dynein, catalytic RNA, and telomerase. This volume is a comprehensive resource for using Tetrahymena in the laboratory and is aimed at persons already experienced, as well as newcomers to the organism. It covers both the biological background and essential protocols for investigators rapidly turning to Tetrahymena as the experimental system of choice.
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Tetrahymena thermophila is a powerful experimental organism that combines a biological complexity similar to that of metazoans (including mammals) with the experimental accessibility of a protist. Past investigations with Tetrahymena have yielded several key discoveries, including dynein, catalytic RNA, and telomerase. Recent developments now enable the investigator to replace any gene by homologous recombination and evaluate the resulting phenotype in a living cell. This book is aimed at persons who are familiar with Tetrahymena as well as newcomers to the organism. By providing both the biological background and the important protocols in one volume, this book is essential to investigators rapidly turning to Tetrahymena as the experimental system of choice.
* Contains both theoretical and practical issues in 30 chapters contributed by the world authorities on Tetrahymena
* Overviews the history, cell biology, and genetics of the organism
* Describes essential protocols on the growth of cells, genetic techniques, and how to look at the cells with the microscope
* Illustrates how the methods can be applied to solve various cell biological problems
* Reviews recently developed strategies for altering gene expression
David Asai received his Ph.D. in biology from the California Institute of Technology in 1980. After postdoctoral research at Caltech and the University of California, Santa Barbara, he served for two years as a Research Biologist at UCSB. In 1985, he moved to Purdue University where he is now Professor of Biological Sciences. His teaching duties include the large introductory cell biology course for undergraduate majors and advanced laboratory courses in cell and molecular biology and fluorescence microscopy.
James Forney received a Ph.D. in Molecular Biology at Indiana University. In 1988, after postdoctoral work at the University of California, Berkeley, he joined the faculty at Purdue University where he is now Professor of Biochemistry. Throughout his career, James Forney has maintained an interest in gene expression and DNA rearrangements in ciliated protozoa. His current teaching responsibilities include general biochemistry and a graduate course in nucleic acid biochemistry.
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