Green Fluorescent Proteins, Volume 58 (Methods in Cell Biology, Volume 58)

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9780126760750: Green Fluorescent Proteins, Volume 58 (Methods in Cell Biology, Volume 58)

This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods.

Key Features
* Only volume on Green Fluorescent Protein research
* Covers all aspects of GFP
* Provides specific microscopic and data analysis methods
* Discusses the design and construction of GFP fusion proteins
* Covers GFP expression in animals, insects, plants, and microbes
* Details procedures for time lapse imaging of living cells
* Explains how to implement single molecule fluorescence detection with GFP
* Discusses dual label GFP strategies for multicolor fluorescence
* Presents fluorescence resonance energy transfer methods with GFPs
* Details quantitative fluorescence imaging techniques
* Extensively illustrated with color photographs

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From the Back Cover:

The green fluorescent Protein (GFP) has initiated a revolution in molecular cell biology by establishing a general approach for visualizing nearly any protein of interest in any cell, tissue or species. Researchers working at all levels of biology, such as single molecule dynamics, protein trafficking within cells, organelle dynamics and cell and tissue behaviors during development, have made important use of GFP.
This volume is a practical guide to working with GFP and contains discussions of GFP biophysics and GFP mutants, design of GFP fusion proteins and expression systems, and detailed protocols for introducing and visualizing GFP in living cells. Methods for quantitative imaging, data analysis and presentation are detailed by a select group of researchers who have made some of the key contributions to bio-fluorescent imaging technologies.
Key Features
* Discusses the design and construction of GFP fusion proteins
* Covers GFP expression in animals, insects, plants, and microbes
* Details procedures for time-lapse imaging of living cells
* Explains how to implement single-molecule fluorescence detection with GFP
* Discusses dual-label GFP strategies for multicolor fluorescence
* Presents fluorescence resonance energy transfer methods with GFPs
* Details quantitative flourescence imagine techniques
* Extensively illustrated with color photographs

About the Author:

Edited by Leslie I. Wilson, Paul T. Matsudaira, Kevin F. Sullivan, and SteveA. Kay

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Kevin F. Sullivan (Editor), Steve A. Kay (Editor), Leslie Wilson (Series Editor), Paul T. Matsudaira (Series Editor)
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