In the 1980s, capillary electrophoresis (CE) joined high-performance liquid chromatography (HPLC) as the most powerful separation technique available to analytical chemists and biochemists. Published research using CE grew from 48 papers in the year of commercial introduction (1988) to 1200 in 1997. While only a dozen major pharmaceutical and biotech companies have reduced CE to routine practice, the applications market is showing real or potential growth in key areas, particularly in the DNA marketplace for genomic mapping and forensic identification. For drug development involving small molecules (including chiral separations), one CE instrument can replace 10 liquid chromatographs in terms of speed of analysis. CE also uses aqueous rather than organic solvents and is thus environmentally friendlier than HPLC. The second edition of Practical Capillary Electrophoresis has been extensively reorganized and rewritten to reflect modern usage in the field, with an emphasis on commercially available apparatus and reagents. This authoritative and very comprehensible treatment builds on the author's extensive experience as an instructor of short courses for the American Chemical Society and for industry.
* Illustrated with detailed diagrams of electrophoretic phenomena
* Offers step-by-step methods development schemes
* Presents techniques for developing quantitative, robust, and precise methods
* Includes an extensive troubleshooting guide
* Updates and greatly expands on the first edition-more than 50% of the text is new
* Written by an internationally recognized scientist who is an instructor for American Chemical Society short courses on HPCE
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High-performance capillary electrophoresis (HPCE) has progressed from a research tool to the technique of choice for solving a variety of separation problems. Practical Capillary Electrophoresis, Second Edition , takes the "black art" out of HPCE by providing an integrated, intuitive, and phenomenological approach to the workings of electrophoresis, selection of the mode of electrophoresis, methods development, validation, and troubleshooting. Instructions in the text will yield the quickest means of producing optimal and highly reproducible separations regardless of which mode of HPCE is selected. Written for both new and experienced users of HPCE, this comprehensive and well-illustrated text is ideal for analytical chemists and biochemists working on applications from small molecules and chiral recognition to peptides, proteins, and DNA.About the Author:
Robert Weinberger, CE Technologies, Chappaqua, New York
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