This volume of the International Review of Neurobiology was written to assist researchers without any previous experience with in situ hybridization, allowing them to follow the protocols and expect good results. It contains all the information required for newcomers to achieve successful in situ hybridization results, and methods for improving the technique of those already utilizing it.
Published since 1959, International Review of Neurobiology is a well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists. Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research.
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Focusing on the vertebrate brain, the new edition of this successful book contains all of the information required for newcomers to achieve successful in situ hybridization results and will enable those already using the technique to improve their methodology.
Fully updated and revised to take into account recent developments, this book provides user-friendly, clear, detailed protocols together with helpful illustrations and troubleshooting tips. Part I describes the well tried and tested technique using radioactive labelled oligonucleotides and Part II covers non-radioactive in situ hybridization methods.
New chapters include: cryostat sectioning of brains; processing of retinal tissue for in situ hybridization; processing the spinal cord for in situ hybridization; processing human brain tissue for in situ hybridization; and, non-radioactive in situ hybridization: simplified procedures for use in whole mounts of mouse and chick embryos.About the Author:
W. Wisden, MRC Laboratory of Molecular Biology, Cambridge, U.K. B. J. Morris, University of Glasgow, Scotland, U.K.
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