Microbiology Lab Manual (2nd Edition)

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9780130100290: Microbiology Lab Manual (2nd Edition)

? This manual serves as a general introduction to the microbiology laboratory, including basic procedures and equipment. Its 36 stand-alone exercises include explanations of the salient points being demonstrated or tested, and are divided into nine sections—Microscopic Technique, Microbial Diversity, Microbial Cultivation Techniques, Identification Techniques, Microbial Growth, Microbial Control, Clinical Microbiology, Virology, and Applied Microbiology. Questions are provided with each exercise to reinforce users' understanding of basic concepts, and require them to analyze or apply the material under discussion. For use with any standard microbiology textbook.

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This laboratory manual recognizes that microbiology students need to establish a firm foundation in underlying principles as well as participate in realistic and practical experiments. Each laboratory exercise report form includes questions relating to the laboratory exercise that require relating background material to the test results, interpretation of the test results, and critical thinking/application questions that require the student to draw upon their own experience.

Topics Include:
  • Preparation of Smears and Simple Stains
  • Ubiquity of Microorganisms
  • Isolation Techniques
  • Detection of Mutant Strains of Bacteria
  • Epidemiology
  • Water Analysis

Excerpt. © Reprinted by permission. All rights reserved.:

ABOUT THE MANUAL

This manual has been written for the Introductory Microbiology student enrolled in either a general or an allied health program. It is intended for those students who take microbiology during their freshman or sophomore year and who, typically, have had only one year (or less) of chemistry. Its aim is to familiarize the student with the basic procedures and equipment used in the microbiology laboratoryincluding—diagnostic microbiology, clinical sample and sampling, serological procedures, antibiotic sensitivity testing and so on.

The manual has several important features. Each exercise has been written to be reasonably self-contained. It features explanations of the salient points being demonstrated or tested. These explanations are meant to supplement and complement, not replace, the assigned text. The exercises have been prepared in a manner that will provide flexibility in their application, dependent on the time frame of the laboratory. This will also enable the exercise to be divided up for performance by individual members of the laboratory group.

DOING YOUR PART

Each exercise is designed to teach a new technique or illustrate a principle of microbial growth. The exercises are arranged in the -following sequence:

  • Background material discussing the theory and experimental design underlying the procedures used in the exercise;
  • Objectives of the laboratory exercise; Materials needed for the laboratory;
  • Procedures outlining the specific instructions for completing the laboratory exercise; and
  • Laboratory Report Form.

As you proceed through the manual, you may notice that the laboratory exercises get progressively more complex and the instructions get somewhat less comprehensive. You are expected to become more competent with each exercise, and you should be able to problem-solve some of the needed procedures for yourself.

Laboratory reports are required for all exercises, and report forms are included for each exercise. They require tabulation of the data acquired in the exercise and include questions based on the experimental procedures and principles demonstrated in the exercise. Many of the questions will require your application of the observed procedures or results to other situations.

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