Cell Separation: A Practical Approach (Practical Approach Series) - Softcover

Softcover

ISBN 10: 019963579X ISBN 13: 9780199635795

Publisher: Oxford University Press, 1999

Synopsis

The successful separation of specific cells requires not only a knowledge of potential methods, but also detailed protocols that are known to work well in practice. This book is unique in that it brings together the best methods currently used for separating cells. For each approach, the authors provide clear explanations of the theoretical basis of the procedures as well as practical details of the technique.
The first chapter describes the isolation of single cell suspensions from animal tissues and from adherent cell culture. It includes the use of lavages, problems of extracellular matrix, methods of assessing cell viability, and the separation of viable from non-viable cells. The following seven chapters discuss specific methods for the separation of cells and the fractionation of cell populations. Sedimentation methods, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, as well as numerous other procedures are discusses as well.
This book should be read by all researchers for whom cell separation is the starting point for their experimental work. As such, it is an invaluable laboratory manual for cell biologists, biochemists, molecular biologists, and physiologists.

About this title

About the Author

Derek Fisher is at Royal Free Hospital School of Medicine. Gillian E. Francis is at Royal Free Hospital School of Medicine.

Review

"In this reference British researchers have contributed articles covering the process of cell separation using a number of different methods, with explanations of the theoretical bases for these procedures. The chapters explain when a particular method would be best used, based on cell size, density, surface charge, hydrophobic surface properties, and antigen status. The methods described include sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immuno methods (including magnetic, column, and panning techniques), and free flow electrophoresis."--SciTech Book News

"The title of this book describes the contents within, and reviews a subject that is underrepresented in the literature. The seven chapters discuss the preparation of single cell suspensions and six typical separation methods: sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immunomethods (magnetic, column, and panning techniques), and free flow electrophoresis. The descriptions emphasize practical applications of these techniques including protocols for most, if not all methods. Although the theoretical basis of these techniques are not heavily emphasized, adequate references are given for those interested."--The Quarterly Review of Biology

Edition details

• Publisher: Oxford University Press
• Publication date: 1999
• ISBN 10: 019963579X
• ISBN 13: 9780199635795
• Binding: Paperback
• Language: English
• Edition number: 1
• Number of pages: 286
• Editor: Fisher Derek, Francis Gillian E., Rickwood David