Lymphocytes: A Practical Approach (Practical Approach Series) - Softcover

Softcover

ISBN 10: 0199638160 ISBN 13: 9780199638161

Publisher: Oxford University Press, 2000

Synopsis

Cellular immunology is a rapidly moving field in which recent advances have made significant contributions to our understanding of the immune response to infection and malignancy. These in turn, have given rise to new therapeutic opportunities in areas such as vaccines and immunotheraphy. Many investigators have been discourages by the complicated protocols involved in cellular immunological studies, as illustrated, by the meticulous care required for the generation of antigen-specific T-cells. Lymphocytes: A Practical Approach (second edition) contains straight-forward protocols for well- established procedures in the study of lymphocytes including preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification assays. It also covers the recent technological advances which have revolutionised the field, such as the use of the Interferon-gamma ELISpot assay and peptide-HLA tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing.

About this title

About the Author

Sarah L. Rowland-Jones is at Institute of Molecular Medicine, Oxford. Andrew McMichael is at Institute of Molecular Medicine, Oxford.

Edition details

• Publisher: Oxford University Press
• Publication date: 2000
• Language: English
• ISBN 10: 0199638160
• ISBN 13: 9780199638161
• Binding: Paperback
• Edition number: 2
• Number of pages: 352
• Editor: Rowland-Jones Sarah L., McMichael Andrew J.