Paul C. Sadek The HPLC Solvent Guide

ISBN 13: 9780471118558

The HPLC Solvent Guide

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9780471118558: The HPLC Solvent Guide
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An indispensable working resource for anyone who performs liquid chromatography

The HPLC Solvent Guide provides an opportunity for analytical chemists to expand their experimental toolkits.

It features extensive and detailed coverage of HPLC solvents currently used in most laboratory settings. Over the course of seven chapters, each one focusing on a specific solvent class, the author provides all the practical information needed to develop separations using these solvent classes. A valuable working reference for experimentalists in a broad array of industrial and research fields, The HPLC Solvent Guide
* Divides solvents according to chemical class, then arranges the methods by field of application, and finally by groups of analytes
* Provides complete descriptions of separations, including sample matrices, lists of analytes separated and quantified, chromatographic parameters, and results
* Features tables listing individual solvents and their physical, chemical, and chromatographic properties; safety and health parameters; and availability, both in terms of supplier and performance specifications
* Includes figures that show the chemical structure of each solvent discussed

Solvent selection is perhaps the most commonly overlooked of experimental parameters in high-performance liquid chromatography. When performing separations, even the most experienced analytical chemist tends to select one of three solvents-acetonitrile, methanol, or water-overlooking the fact that many different classes of solvent can be used very effectively in HPLC. By providing extensive detailed coverage of HPLC solvents currently used in a wide range of separations, The HPLC Solvent Guide offers chemists an opportunity to expand their experimental repertoires.

The author begins with an in-depth review of the role of solvents in HPLC. This is followed by a series of chapters devoted to the different classes of solvent. To facilitate easy reference, chapters are first grouped by solvent classes, including alcohols, alkanes, ethers, ketones, and nitriles. They are then further divided by field of application (e.g., environmental, pharmaceutical) and by specific analyte class (e.g., priority pollutants, anticancer drugs). Throughout, each separation is described as fully as possible, listing the sample matrix, analytes separated and quantified, chromatographic parameters used, and abbreviated results. As a consequence, even novice chromatographers have sufficient information to begin to develop a separation on the basis of a citation alone.

Appealing to anyone doing HPLC are the four tables appearing in each of the seven chapters dealing with individual solvent classes. These tables list physical, chemical, and chromatographic properties; safety and health parameters; and availability, both in terms of supplier and performance specifications. A separate table provides the chemical structures of the solvents presented in each chapter.

In writing this book, the author has drawn heavily from contemporary references in which the chromatograms for described separations were included, thus providing readers with clear reference points from which to conduct a more extensive historical literature review, if needed.

Providing the most detailed treatment of HPLC solvents to date, The HPLC Solvent Guide is a valuable working resource for anyone who performs liquid chromatography.

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From the Publisher:

This practical work offers experimentalists an overview of the solvent's role in HPLC as well as detailed discussions of seven different solvent classes used in connection with HPLC methods. Each chapter on individual solvent classes includes a series of four tables which compile physical, chemical, and chromatographic properties; safety and health parameters; chemical structures; and availability, in terms of both supplier and performance specifications. The solvents are grouped into primary classes, followed by type of application and then by specific analyte class. Every separation is described in-depth including the sample matrix, list of analytes (separated and quantitated), chromatographic parameters used and abbreviated results.

From the Inside Flap:

* Reviews the role of solvents in HPLC.
* Detailed and expanded presentation reviewing all commonly-used solvent classes and chemical and physical properties.
* Easy reference for solvent class, field of application, and specific analyte class.
* Each separation is described fully; listing sample matrix, analytes separated and quantified, chromatographic parameters used, and abbreviated results.
* Popular "how to" and "how not to" methodology.
* Tables feature physical, chemical, and chromatographic properties of individual solvent classes; safety and health parameters; availability in terms of supplier and performance specifications; and chemical structures of solvents.
* Focus is on practical applications and use of method optimization processes.

New to the 2nd edition:
* Detailed discussion of method optimization techniques (triangulation, window diagrams).
* The concept of method validation is presented, including functional definitions and general guidelines for validation protocols.
* Detailed discussion of system suitability parameters, what they are, and how they are used.
* Over 600 new abstracted articles reviewed, building on 1,100 articles in the 1st edition.
* A key area of increased focus is natural products/botanicals/herbals.

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9780471411383: The HPLC Solvent Guide

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ISBN 10:  0471411388 ISBN 13:  9780471411383
Publisher: Wiley, 2002
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