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Here is a step-by-step guide to the general methods used for protein research. For each method, all reagents and items of equipment are described; a detailed protocol is presented; and potential problems, and pitfalls are discussed. Basic methods for extracting, handling, storing and measuring the concentration of proteins are presented. Various approaches to the study of proteins, using gel electrophoresis and related methods, are also covered. It will allow a novice to perform the various procedures. Experienced research workers will find critical information concerning advanced procedures assembled in a single source for the first time.
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Protein Methods by Daniel M. Bollag, Michael D. Rozycki, Stuart J. Edelstein
Reviews from the first edition
"...it is very well written...would certainly be of use to undergraduate and postgraduate students entering the complex world of protein preparation and to more experienced scientists entering this field for the first time." --Biotechnology and Applied Biochemistry
"The book presents these topics with loads of practical detail, so that the reader has little need to consult other reference sources to carry out the techniques described. All in all, a useful book..." --Theoretical & Applied Genetics
"...clearly written with protocols that are easy to follow and the text is well spread-out and easy to read...all methods are fully referenced...a useful book for beginners at a reasonable price." --FEBS Letters
This revised and expanded Second Edition of Protein Methods remains the first source for a complete summary of tested and proven protein techniques. Now divided into two parts, the book begins with the essential chapters from the first edition, updated to reflect important changes in methodology. The chapter on protein isolation includes a new section focusing on the isolation of proteins from inclusion bodies. In the second section, four new chapters are devoted to protein purification and crystallization.
* Preparation for Protein Isolation
* Protein Extraction and Solubilization
* Protein Concentration Determination
* Concentrating Protein Solutions
* Gel Electrophoresis Under Denaturing Conditions
* Gel Electrophoresis Under
* reagent items and equipment required for each method
* chapter "view-at-a-glance" tabs
* illustrations for all gel applications and other methods
* complete supplier list
* appendices of basic chemical parameters and molecular data
* key references
* descriptive tables
* Safety notes.
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Book Description John Wiley & Sons, 1990. Condition: Good. Spiral. Shows some signs of wear, and may have some markings on the inside. Seller Inventory # GRP2554933
Book Description John Wiley & Sons, 1990. Condition: Good. Spiral. Former Library book. Shows some signs of wear, and may have some markings on the inside. Seller Inventory # GRP69774703
Book Description Wiley & Sons, Incorporated, John. Paperback. Condition: Good. A copy that has been read, but remains in clean condition. All pages are intact, and the cover is intact. The spine may show signs of wear. Pages can include limited notes and highlighting, and the copy can include previous owner inscriptions. An ex-library book and may have standard library stamps and/or stickers. At ThriftBooks, our motto is: Read More, Spend Less. Seller Inventory # G0471568716I3N10
Book Description Wiley-Liss, Inc., New York, 1991. Paperback. Condition: Good. Ring binding paperback. Scores on covers. Sticker on front cover. Spiral cover is torn and reinforced with tape. Corners and edges are worn. Rear upper leading corner is slightly creased. Contents are clean and clear. AM. Used. Seller Inventory # 416452
Book Description Wiley-Blackwell, 1991. Condition: Good. This is an ex-library book and may have the usual library/used-book markings inside.This book has soft covers. In good all round condition. Spiral Bound. Seller Inventory # 7372614
Book Description Wiley-Liss, 1991. Paperback. Condition: Used: Good. Seller Inventory # SONG0471568716
Book Description Wiley, 1990. Paperback. Condition: Good. Ships with Tracking Number! INTERNATIONAL WORLDWIDE Shipping available. May not contain Access Codes or Supplements. May be ex-library. Shipping & Handling by region. Buy with confidence, excellent customer service!. Seller Inventory # 0471568716