Protein Methods

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9780471568711: Protein Methods
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Here is a step-by-step guide to the general methods used for protein research. For each method, all reagents and items of equipment are described; a detailed protocol is presented; and potential problems, and pitfalls are discussed. Basic methods for extracting, handling, storing and measuring the concentration of proteins are presented. Various approaches to the study of proteins, using gel electrophoresis and related methods, are also covered. It will allow a novice to perform the various procedures. Experienced research workers will find critical information concerning advanced procedures assembled in a single source for the first time.

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From the Back Cover:

Protein Methods by Daniel M. Bollag, Michael D. Rozycki, Stuart J. Edelstein

Reviews from the first edition
"...it is very well written...would certainly be of use to undergraduate and postgraduate students entering the complex world of protein preparation and to more experienced scientists entering this field for the first time." --Biotechnology and Applied Biochemistry

"The book presents these topics with loads of practical detail, so that the reader has little need to consult other reference sources to carry out the techniques described. All in all, a useful book..." --Theoretical & Applied Genetics

"...clearly written with protocols that are easy to follow and the text is well spread-out and easy to read...all methods are fully referenced...a useful book for beginners at a reasonable price." --FEBS Letters

This revised and expanded Second Edition of Protein Methods remains the first source for a complete summary of tested and proven protein techniques. Now divided into two parts, the book begins with the essential chapters from the first edition, updated to reflect important changes in methodology. The chapter on protein isolation includes a new section focusing on the isolation of proteins from inclusion bodies. In the second section, four new chapters are devoted to protein purification and crystallization.

Chapters include
* Preparation for Protein Isolation
* Protein Extraction and Solubilization
* Protein Concentration Determination
* Concentrating Protein Solutions
* Gel Electrophoresis Under Denaturing Conditions
* Gel Electrophoresis Under
...and feature
* reagent items and equipment required for each method
* chapter "view-at-a-glance" tabs
* illustrations for all gel applications and other methods
* complete supplier list
* appendices of basic chemical parameters and molecular data
* key references
* descriptive tables
* Safety notes.

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Other Popular Editions of the Same Title

9780471118374: Protein Methods

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ISBN 10:  0471118370 ISBN 13:  9780471118374
Publisher: Wiley-Liss, 1996
Softcover

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Bollag, Daniel M., Edelstein, Stuart J.
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