Green Fluorescent Protein: Properties, Applications and Protocols (Methods of Biochemical Analysis)

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9780471736820: Green Fluorescent Protein: Properties, Applications and Protocols (Methods of Biochemical Analysis)
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Since the discovery of the gene for green fluorescent protein(GFP), derived from jellyfish, this protein that emits a green glowhas initiated a revolution in molecular biosciences. With thistool, it is now possible to visualize nearly any protein ofinterest in any cell or tissue of any species. Since thepublication of the first edition, there have been tremendouslysignificant technological advances, including development of newmutant variants. Proteins are now available in yellow and blue, andNovel Fluorescent Proteins (NFPs) have expanded their utility indeveloping biosensors, biological markers, and other biologicalapplications.

This updated, expanded new edition places emphasis on the rise ofNFPs, including new chapters on NFP properties with detailedprotocols, applications of GFPs and NFPs in industry research, andbiosensors. This book provides a solid theoretical framework, alongwith detailed, practical guidance on use of GFPs and NFPs withdiscussion of potential pitfalls. The expert contributors providereal examples in showing how to tailor GFP/NFP to specific systems,maximize expression, and enhance detection.

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From the Publisher:

Green fluorescent protein (GFP) has become a significant and widely used reporter system for in vivo studies that cut across the boundaries of biochemistry, physiology, and cell, developmental and molecular biology. This book provides the first collection of studies examining the versatile uses and applications of this reporter system.

From the Back Cover:

The latest edition of the definitive text in the field offluorescent proteins

Green fluorescent protein (GFP), a biological marker cloned fromjellyfish, has emerged from virtual obscurity to the forefront ofbiomolecular research in just a few years. GFP and relatedfluorescent proteins (FPs) have been intensely studied—thesubjects of hundreds of in vivo projects worldwide. These studieshave resulted in new applications and published articles, emergingat a breathtaking rate. The success of FPs and their many usespromise to make them some of the most powerful tools inbiotechnology, pharmaceutical science, and molecular and cellularbiology.

Written by pioneers in the field, this text tackles both theoryand practice, offering numerous case studies, examples,illustrations, and troubleshooting tips. It examines how FPs aretailored for specific systems and used to maximize expression, andhow variants are generated with altered properties. The text alsoexplores new ways to use FPs and methods for enhancing detection ina variety of organisms and cell types.

This updated and expanded edition places emphasis on the rise ofFPs and their applications in industry and biosensor research.Green Fluorescent Protein: Properties, Applications, andProtocols, Second Edition provides: New chapters on FPs,biosensors, and advances in the use of FPs in the biotechnology andpharmaceutical industries Detailed information about protocolsusing FPs Examples from recent notable studies Background on thebiochemical and physical properties, three-dimensional structure,and molecular biology and mutation of FPs The application of FPsacross organisms

Green Fluorescent Protein: Properties, Applications, andProtocols, Second Edition is essential reading for researchersand graduate students in molecular and cellular biology,physiology, biochemistry, developmental biology, neuroscience,ecology, in vivo biology, and plant molecular biology.

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9780471178392: Green Fluorescent Protein: Properties, Applications, and Protocols

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ISBN 10:  047117839X ISBN 13:  9780471178392
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9780471453284: Green Fluorescent Protein: Properties, Applications, And Protocols (Methods of Biochemical Analysis)

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Book Description John Wiley and Sons Ltd, United States, 2005. Hardback. Condition: New. 2nd Edition. Language: English. Brand new Book. Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection. Seller Inventory # AAH9780471736820

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Book Description John Wiley and Sons Ltd, United States, 2005. Hardback. Condition: New. 2nd Edition. Language: English. Brand new Book. Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection. Seller Inventory # AAH9780471736820

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