Gene Cloning and Manipulation

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9780521407007: Gene Cloning and Manipulation

This text gives a broad, but concise, coverage of gene cloning and manipulation, suitable for undergraduates and beginning graduate students. Assuming only general biochemical knowledge, it stresses the concepts underlying particular types of cloning vector, and uses examples to illustrate them, rather than simply presenting a mass of detailed lists and vector maps. The book starts by describing the principles behind cloning DNA in E. coli, the enzymes used, the range of cloning vectors available, and how to screen libraries to find particular clones. The author shows how PCR can be used as an alternative, or complementary, approach. He then goes on to describe how sequences can be exploited, after cloning and identification, by site-directed mutagenesis and over-expression. The book finishes with a detailed presentation of the genetic manipulation of other organisms, including other bacteria, yeast, plants, insects, and mammals.

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Book Description:

Genetic engineering underlies most modern biological research. This book explains the methods, stressing the underlying principles, to undergraduates and new researchers. It covers the genetic manipulation of animals and plants as well as micro-organisms and combines broad coverage with a concise, but thorough, treatment.

About the Author:

Christopher Howe is Professor of Plant and Microbial Biochemistry at the University of Cambridge. His research interests include biochemistry and molecular biology of photosynthetic organisms, and the evolutionary processes that gave rise to them. He has taught molecular biology for twenty years.

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Christopher Howe
Published by Cambridge University Press (1995)
ISBN 10: 0521407001 ISBN 13: 9780521407007
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Christopher Howe
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