Purifying Proteins for Proteomics: A Laboratory Manual

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9780879696962: Purifying Proteins for Proteomics: A Laboratory Manual
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This substantial text provides researchers with detailed descriptions of laboratory methods used to prepare proteins for proteomic analysis using chromatography and electrophoresis. Six chapters are also included for the analysis of purified proteins prior to structural proteomics, with topics including analytical ultracentrifugation, measuring conformational stability, protein characterization with mass spectrometry, and analyzing carbohydrate from glycoproteins. Each chapter is clearly organized in outline form and includes discussion, methods, troubleshooting, and protocols. Appendices are provided that detail protein structure, measuring concentration of proteins, concentrating solutions, protein stabilization for storage, one-dimensional polyacrylamide gel electrophoresis, and troubleshooting guides and cautions. The manual will accompany Proteins and proteomics by the same author. Simpson, who authored many of the chapters as well as editing the text, is at the Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research in Melbourne, Australia. Annotation 2004 Book News, Inc., Portland, OR (booknews.com)

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9780879696955: Purifying Proteins for Proteomics: A Laboratory Manual

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ISBN 10:  0879696958 ISBN 13:  9780879696955
Publisher: Cold Spring Harbor Laboratory Pr, 2003
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