Antibody Phage Display: Methods and Protocols (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 0896039064 ISBN 13: 9780896039063

Publisher: Humana Press

Synopsis

This comprehensive collection of established antibody phage display protocols features authoritative guidance that will enable the nonspecialist successfully to carry them out. Coverage spans the construction of antibody libraries, the selection of antibody clones with the desired properties, and their modification, expression, and purification. Comprehensive and highly practical, Antibody Phage Display: Methods and Protocols provides biochemists, molecular biologists, and immunologists with a gold-standard reference guide to the successful isolation, modification, and expression of recombinant antibodies using today's powerful phage display technology.

About this title

From the Back Cover

Antibody phage display, the definitive technology for monoclonal production, has now advanced to a stage where it can be performed in nonspecialized research laboratories. In Antibody Phage Display: Methods and Protocols, Philippa M. O'Brien and Robert Aitken combine in one volume a comprehensive collection of established antibody phage display protocols, each accompanied by authoritative guidance that will enable the nonspecialist to carry them out successfully. Coverage spans the construction of antibody libraries, the selection of antibody clones with the desired properties, and their modification, expression, and purification. Each readily reproducible method is described by a hands-on expert in step-by-step detail and includes a wealth of practical advice not found in the scientific literature. Extensive notes discuss pitfalls to avoid and offer many possible alternative methods to suit special research situations. An overview by one of the world's leading experts in antibody phage display, Dr. Hennie R. Hoogenboom, surveys the current status of the field and the future of the technology.
Comprehensive and highly practical, Antibody Phage Display: Methods and Protocols provides biochemists, molecular biologists, and immunologists with a gold-standard reference guide to the successful isolation, modification, and expression of recombinant antibodies using today's powerful phage display technology.

Review

"...this book lives up to its promises. It starts with an excellent introduction by Hennie Hoogenboom, reprinted from his Immunotechnology article, which sets the scene and provides an overview of the field for new readers...Members of the laboratory that used the book for reference found it very clear and all were keen that the review was completed as quickly as possible so that the book was returned to the laboratory where they could make use of it! I think this in itself supports the fact that the book is a useful volume." - Immunology News

"As with other volumes in this popular series, each protocol is discussed in detail, including an introduction, materials, methods notes, and references. It is a good purchase for research libraries where monoclonal antibodies are studied." E-Streams

Edition details

• Publisher: Humana Press
• Language: English
• ISBN 10: 0896039064
• ISBN 13: 9780896039063
• Binding: Hardcover
• Edition number: 1
• Number of pages: 416