In Situ Detection of DNA Damage: Methods and Protocols (Methods in Molecular Biology)

0 avg rating
( 0 ratings by Goodreads )
 
9780896039520: In Situ Detection of DNA Damage: Methods and Protocols (Methods in Molecular Biology)
View all copies of this ISBN edition:
 
 

Detection and analysis of DNA damage is of critical importance in a variety of biological disciplines studying apoptosis, cell cycle and cell di- sion, carcinogenesis, tumor growth, embryogenesis and aging, neu- degenerative and heart diseases, anticancer drug development, environmental and radiobiological research, and others. Individual cells within the same tissue or in cell culture may vary in the extent of their DNA damage and, consequently, can display different re- tions to it. These differences between individual cells in the same cell popu- tion are detected using in situ approaches. In situ is a Latin term meaning “on site” or “in place.” It is used to denote the processes occurring or detected in their place of origin. In mole- lar and cell biology this usually refers to undisrupted mounted cells or tissue sections. In that meaning “in situ” is used as part of the terms “in situ PCR,” “in situ transcription,” “in situ hybridization,” “in situ end labeling,” and “in situ ligation.” Sometimes the “in situ” term is applied at the subcellular level to cells disrupted in the process of analysis, for example, in the detection of specific sequences in chromosomes using fluorescent in situ hybridization (FISH). Historically, the term was used primarily in methods dealing with nucleic acids.

"synopsis" may belong to another edition of this title.

From the Back Cover:

Detection and analysis of DNA damage is critically important in the study of the widest variety of biological phenomena, ranging from apoptosis and aging to heart diseases and anticancer drug development. In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and show how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. In-depth protocols for single-cell electrophoresis (comet assay) are presented, as well as those for the detection of indirect markers of DNA damage and modified bases. Technical reviews discuss specificity, sensitivity, advantages, and limitations of the techniques described, and compare them with alternative approaches.
State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying not only the in situ detection of DNA damage but also the labeling of apoptotic cells.

Review:

"Investigators working in the general area of DNA damage, including apoptosis, will find this book of use. The protocols are sufficiently detailed so as to allow experienced laboratory workers access to the described methods. . .the broad spectrum of methods described allows for individual investigators to exercise options."-Doody's Health Sciences Book Review Journal

"Here, a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa)." - European Journal of Histochemistry

"About this title" may belong to another edition of this title.

Buy New View Book
List Price: US$ 179.00
US$ 20.39

Convert currency

Shipping: US$ 7.19
From United Kingdom to U.S.A.

Destination, rates & speeds

Add to Basket

Top Search Results from the AbeBooks Marketplace

1.

Published by Humana Press
ISBN 10: 0896039528 ISBN 13: 9780896039520
New Quantity Available: 4
Seller:
Majestic Books
(London, ,, United Kingdom)
Rating
[?]

Book Description Humana Press. Condition: New. pp. xvii + 313 , Figures. Seller Inventory # 7629616

More information about this seller | Contact this seller

Buy New
US$ 20.39
Convert currency

Add to Basket

Shipping: US$ 7.19
From United Kingdom to U.S.A.
Destination, rates & speeds

2.

DIDENKO VLADIMIR V
ISBN 10: 0896039528 ISBN 13: 9780896039520
New Quantity Available: 2
Seller:
firstbookstore
(New Delhi, India)
Rating
[?]

Book Description Condition: Brand New. Brand New Original US Edition, Perfect Condition. Printed in English. Excellent Quality, Service and customer satisfaction guaranteed!. Seller Inventory # AINDN-3808

More information about this seller | Contact this seller

Buy New
US$ 32.09
Convert currency

Add to Basket

Shipping: FREE
From India to U.S.A.
Destination, rates & speeds

3.

DIDENKO VLADIMIR V
ISBN 10: 0896039528 ISBN 13: 9780896039520
New Quantity Available: 1
Seller:
Bookshub
(Karol Bagh, India)
Rating
[?]

Book Description Condition: New. New. US edition. Perfect condition. Customer satisfaction our priority. Seller Inventory # ABE-OCT-117357

More information about this seller | Contact this seller

Buy New
US$ 32.42
Convert currency

Add to Basket

Shipping: FREE
From India to U.S.A.
Destination, rates & speeds

4.

DIDENKO VLADIMIR V
ISBN 10: 0896039528 ISBN 13: 9780896039520
New Quantity Available: 1
Seller:
EBOOKSTORE2010
(New Delhi, ND, India)
Rating
[?]

Book Description Condition: Brand New. New. US edition. Customer Satisfaction guaranteed!!. Seller Inventory # SHUB117357

More information about this seller | Contact this seller

Buy New
US$ 32.47
Convert currency

Add to Basket

Shipping: FREE
From India to U.S.A.
Destination, rates & speeds

5.

DIDENKO VLADIMIR V
ISBN 10: 0896039528 ISBN 13: 9780896039520
New Quantity Available: 2
Seller:
Romtrade Corp.
(STERLING HEIGHTS, MI, U.S.A.)
Rating
[?]

Book Description Condition: New. Brand New Original US Edition.We Ship to PO BOX Address also. EXPEDITED shipping option also available for faster delivery.This item may ship fro the US or other locations in India depending on your location and availability. Seller Inventory # AUSBNN-3808

More information about this seller | Contact this seller

Buy New
US$ 34.77
Convert currency

Add to Basket

Shipping: FREE
Within U.S.A.
Destination, rates & speeds

6.

DIDENKO VLADIMIR V
ISBN 10: 0896039528 ISBN 13: 9780896039520
New Quantity Available: 1
Seller:
Basi6 International
(Irving, TX, U.S.A.)
Rating
[?]

Book Description Condition: Brand New. New, US edition. Excellent Customer Service. Seller Inventory # ABEUSAOCT18-117357

More information about this seller | Contact this seller

Buy New
US$ 34.78
Convert currency

Add to Basket

Shipping: FREE
Within U.S.A.
Destination, rates & speeds

7.

DIDENKO VLADIMIR V
ISBN 10: 0896039528 ISBN 13: 9780896039520
New Quantity Available: 2
Seller:
University Bookstore
(DELHI, DELHI, India)
Rating
[?]

Book Description Condition: New. This is Brand NEW. Seller Inventory # Star-18102018-21934

More information about this seller | Contact this seller

Buy New
US$ 26.27
Convert currency

Add to Basket

Shipping: US$ 10.37
From India to U.S.A.
Destination, rates & speeds

8.

Didenko Vladimir V
Published by Humana Press, Germany (2002)
ISBN 10: 0896039528 ISBN 13: 9780896039520
New Hardcover Quantity Available: 10
Seller:
STM Book Stores
(New Delhi, DELHI, India)
Rating
[?]

Book Description Humana Press, Germany, 2002. Hardcover. Condition: Brand New. Book Condition:- Brand New. Secured Packaging. Fast DeliveryBookseller Inventory # 9780896039520. book. Seller Inventory # STM-9780896039520

More information about this seller | Contact this seller

Buy New
US$ 40.16
Convert currency

Add to Basket

Shipping: FREE
From India to U.S.A.
Destination, rates & speeds

9.

Vladimir V. Didenko (Editor)
Published by Humana Press (2002)
ISBN 10: 0896039528 ISBN 13: 9780896039520
New Hardcover Quantity Available: 1
Seller:
Ergodebooks
(RICHMOND, TX, U.S.A.)
Rating
[?]

Book Description Humana Press, 2002. Hardcover. Condition: New. Seller Inventory # DADAX0896039528

More information about this seller | Contact this seller

Buy New
US$ 36.93
Convert currency

Add to Basket

Shipping: US$ 3.99
Within U.S.A.
Destination, rates & speeds

10.

DIDENKO VLADIMIR V
ISBN 10: 0896039528 ISBN 13: 9780896039520
New Quantity Available: 5
Seller:
PLYSH STORE
(South Delhi, DELHI, India)
Rating
[?]

Book Description Condition: Brand New. New Original Edition. Same ISBN as per description. Delivery within 4 - 5 days. Seller Inventory # 0896039528-1102018

More information about this seller | Contact this seller

Buy New
US$ 36.69
Convert currency

Add to Basket

Shipping: US$ 5.19
From India to U.S.A.
Destination, rates & speeds

There are more copies of this book

View all search results for this book