Proteomics of Biological Systems: Protein Phosphorylation Using Mass Spectrometry Techniques

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9781118028964: Proteomics of Biological Systems: Protein Phosphorylation Using Mass Spectrometry Techniques
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Phosphorylation is the addition of a phosphate (PO4) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes, causing or preventing the mechanisms of diseases such as cancer and diabetes. This book shows how to use mass spectrometry to determine whether or not a protein has been correctly modified by the addition of a phosphate group. It also provides a combination of detailed, step-by-step methodology for phosphoproteomic sample preparation, mass spectral instrumental analysis, and data interpretation approaches. Furthermore, it includes the use of bioinformatic Internet tools such as the Blast2GO gene ontology (GO) tool, used to help understand and interpret complex data collected in these studies.

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Obtain protein phosphorylation knowledge using mass spectrometrytechniques

A practical introduction to mass spectrometry–basedphosphoproteomic studies, this book addresses various conceptsincluding the protocols necessary for sample preparation throughmass spectral interpretation of protein phosphorylation events. Itdelves into the determining factors for whether or not a proteinhas been correctly modified by the addition of a phosphate group.Highlighting both basic and significant principles, as well asspecific applications using examples, Proteomics of BiologicalSystems covers:

  • Key fundamentals of mass spectrometry including mass analyzers,ionization, and nano-electrospray/nano-flow liquidchromatography

  • Optimized methodologies for performing post translationalmodification (PTM) of protein studies using tried and tested samplepreparation and analysis methods and approaches

  • Specific examples of eukaryote and prokaryote normal state andperturbed state phosphoproteomic studies

  • Use of the most recent systems biology bioinformatic Internettools for interpreting complex data such as the Blast2GO geneontology tool

  • Step-by-step methodology for phosphoproteomic studies of botheukaryote and prokaryote systems

This unique resource contains specific chapters devoted tonormal state HeLa cell phosphoproteomes, DNA damage response stateHeLa cell phosphoproteomes, Caulobacter crescentus normal statephosphoproteomes, and more. A core guide for scientists andresearchers in academic laboratories and pharmaceutical, chemical,and biotech companies, Proteomics of Biological Systems is a usefultext for expanding comprehension of mass spectrometry–basedphosphoproteomic study.

About the Author:

BRYAN M. HAM, PhD, is a member of the American Society for Mass Spectrometry and the American Chemical Society. He has conducted proteomics and lipidomics research at The Ohio State University and Pacific Northwest National Laboratory in Richland, Washington. He is currently working for the Department of Homeland Security at the U.S. Customs and Border Protection New York Laboratory. He has published numerous research papers in peer-reviewed journals, and is the author of Even Electron Mass Spectrometry with Biomolecule Applications (Wiley).

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Book Description John Wiley & Sons Inc, United States, 2011. Hardback. Condition: New. 1. Auflage. Language: English. Brand new Book. Phosphorylation is the addition of a phosphate (PO 4) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes, causing or preventing the mechanisms of diseases such as cancer and diabetes. This book shows how to use mass spectrometry to determine whether or not a protein has been correctly modified by the addition of a phosphate group. It also provides a combination of detailed, step-by-step methodology for phosphoproteomic sample preparation, mass spectral instrumental analysis, and data interpretation approaches. Furthermore, it includes the use of bioinformatic Internet tools such as the Blast2GO gene ontology (GO) tool, used to help understand and interpret complex data collected in these studies. Seller Inventory # AAH9781118028964

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Book Description John Wiley Sons Inc, United States, 2011. Hardback. Condition: New. 1. Auflage. Language: English . Brand New Book. Phosphorylation is the addition of a phosphate (PO 4) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes, causing or preventing the mechanisms of diseases such as cancer and diabetes. This book shows how to use mass spectrometry to determine whether or not a protein has been correctly modified by the addition of a phosphate group. It also provides a combination of detailed, step-by-step methodology for phosphoproteomic sample preparation, mass spectral instrumental analysis, and data interpretation approaches. Furthermore, it includes the use of bioinformatic Internet tools such as the Blast2GO gene ontology (GO) tool, used to help understand and interpret complex data collected in these studies. Seller Inventory # AAH9781118028964

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Book Description John Wiley & Sons Inc, United States, 2011. Hardback. Condition: New. 1. Auflage. Language: English. Brand new Book. Phosphorylation is the addition of a phosphate (PO 4) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes, causing or preventing the mechanisms of diseases such as cancer and diabetes. This book shows how to use mass spectrometry to determine whether or not a protein has been correctly modified by the addition of a phosphate group. It also provides a combination of detailed, step-by-step methodology for phosphoproteomic sample preparation, mass spectral instrumental analysis, and data interpretation approaches. Furthermore, it includes the use of bioinformatic Internet tools such as the Blast2GO gene ontology (GO) tool, used to help understand and interpret complex data collected in these studies. Seller Inventory # BTE9781118028964

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