Helicobacter pylori Protocols (Methods in Molecular Medicine) - Softcover

Softcover

ISBN 10: 1617370207 ISBN 13: 9781617370205

Publisher: Humana, 2010

Synopsis

Helicobacter pylori Protocols offers an outstanding collection of state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for the culturing of H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed by leading practitioners to solve the difficult technical problems created by the application of the powerful bacterial genetic and molecular cloning techniques to H. pylori.

About this title

From the Back Cover

In Helicobacter pylori Protocols, leading experts bring together an outstanding collection state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for culturing H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed to solve the difficult technical problems created by the application of today's powerful bacterial genetic and molecular cloning techniques to H. pylori.

Helicobacter pylori Protocols contains a wide variety of stand-alone techniques that are unfailingly reproducible. The book is certain to become a standard experimental and clinical laboratory reference for all those engaged in H. pylori work today.

Review

"The book was designed to provide up-to-date detailed protocols for the experienced either working
in the field of Helicobacter research or wishing to enter the field. The authors share their experience and give advice about how to avoid pitfalls and problems encountered with this organism. The book is recommended for those with a serious interest in microbiology and molecular biology of Helicobacter pylori. - David Graham, MD, Baylor College of Medicine

Edition details

• Publisher: Humana
• Publication date: 2010
• ISBN 10: 1617370207
• ISBN 13: 9781617370205
• Binding: Paperback
• Language: English
• Number of pages: 287
• Editor: Clayton Christopher L., Mobley Harry L. T.