Bacterial Cell Surfaces: Methods and Protocols (Methods in Molecular Biology)

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9781627032445: Bacterial Cell Surfaces: Methods and Protocols (Methods in Molecular Biology)

In recent years, molecular microbiology has emerged as a top, cutting-edge biological discipline, thanks to the multi-disciplinary and integrative approaches taken by investigators seeking to understand the intricacies of the microbial world and how it affects human health and the biosphere. In Bacterial Cell Surfaces: Methods and Protocols, recent advances in structural biology, proteomics, and imaging techniques, together with the traditional biochemical and genetic approaches, are provided in order to present an exciting look into the structure, function, and regulation of the bacterial cell envelope. The detailed volume contains examples of traditional and innovative tools for the study of protein structure and function and enzymatic activities, the purification and analysis of macromolecules and their complexes, and the investigation of regulatory mechanisms and cell biological processes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Bacterial Cell Surfaces: Methods and Protocols is aimed at the microbiologist, biochemist, or cell biologist, whether a beginning graduate student or a veteran experimentalist, who wishes to learn new methodologies and take advantage of the years of research and protocol optimization from the best laboratories around the world.

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Published by Humana Press Inc., United States (2013)
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Book Description Humana Press Inc., United States, 2013. Hardback. Book Condition: New. 2013 ed.. Language: English . Brand New Book. In recent years, molecular microbiology has emerged as a top, cutting-edge biological discipline, thanks to the multi-disciplinary and integrative approaches taken by investigators seeking to understand the intricacies of the microbial world and how it affects human health and the biosphere. In Bacterial Cell Surfaces: Methods and Protocols, recent advances in structural biology, proteomics, and imaging techniques, together with the traditional biochemical and genetic approaches, are provided in order to present an exciting look into the structure, function, and regulation of the bacterial cell envelope. The detailed volume contains examples of traditional and innovative tools for the study of protein structure and function and enzymatic activities, the purification and analysis of macromolecules and their complexes, and the investigation of regulatory mechanisms and cell biological processes. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bacterial Cell Surfaces: Methods and Protocols is aimed at the microbiologist, biochemist, or cell biologist, whether a beginning graduate student or a veteran experimentalist, who wishes to learn new methodologies and take advantage of the years of research and protocol optimization from the best laboratories around the world. Bookseller Inventory # LIB9781627032445

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Book Description Humana Press Inc., United States, 2013. Hardback. Book Condition: New. 2013 ed.. Language: English . Brand New Book. In recent years, molecular microbiology has emerged as a top, cutting-edge biological discipline, thanks to the multi-disciplinary and integrative approaches taken by investigators seeking to understand the intricacies of the microbial world and how it affects human health and the biosphere. In Bacterial Cell Surfaces: Methods and Protocols, recent advances in structural biology, proteomics, and imaging techniques, together with the traditional biochemical and genetic approaches, are provided in order to present an exciting look into the structure, function, and regulation of the bacterial cell envelope. The detailed volume contains examples of traditional and innovative tools for the study of protein structure and function and enzymatic activities, the purification and analysis of macromolecules and their complexes, and the investigation of regulatory mechanisms and cell biological processes. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bacterial Cell Surfaces: Methods and Protocols is aimed at the microbiologist, biochemist, or cell biologist, whether a beginning graduate student or a veteran experimentalist, who wishes to learn new methodologies and take advantage of the years of research and protocol optimization from the best laboratories around the world. Bookseller Inventory # LIB9781627032445

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Book Description Humana Press Inc., United States, 2013. Hardback. Book Condition: New. 2013 ed.. Language: English . This book usually ship within 10-15 business days and we will endeavor to dispatch orders quicker than this where possible. Brand New Book. In recent years, molecular microbiology has emerged as a top, cutting-edge biological discipline, thanks to the multi-disciplinary and integrative approaches taken by investigators seeking to understand the intricacies of the microbial world and how it affects human health and the biosphere. In Bacterial Cell Surfaces: Methods and Protocols, recent advances in structural biology, proteomics, and imaging techniques, together with the traditional biochemical and genetic approaches, are provided in order to present an exciting look into the structure, function, and regulation of the bacterial cell envelope. The detailed volume contains examples of traditional and innovative tools for the study of protein structure and function and enzymatic activities, the purification and analysis of macromolecules and their complexes, and the investigation of regulatory mechanisms and cell biological processes. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bacterial Cell Surfaces: Methods and Protocols is aimed at the microbiologist, biochemist, or cell biologist, whether a beginning graduate student or a veteran experimentalist, who wishes to learn new methodologies and take advantage of the years of research and protocol optimization from the best laboratories around the world. Bookseller Inventory # LIE9781627032445

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Book Description Book Condition: New. Publisher/Verlag: Springer, Berlin | Methods and Protocols | This Methods in Molecular Biology(TM) book gathers tools for studying protein structure and function and enzymatic activities, purifying and analyzing of macromolecules and their complexes and investigating regulatory mechanisms and cell biological processes. | In recent years, molecular microbiology has emerged as a top, cutting-edge biological discipline, thanks to the multi-disciplinary and integrative approaches taken by investigators seeking to understand the intricacies of the microbial world and how it affects human health and the biosphere. In Bacterial Cell Surfaces: Methods and Protocols , recent advances in structural biology, proteomics, and imaging techniques, together with the traditional biochemical and genetic approaches, are provided in order to present an exciting look into the structure, function, and regulation of the bacterial cell envelope. The detailed volume contains examples of traditional and innovative tools for the study of protein structure and function and enzymatic activities, the purification and analysis of macromolecules and their complexes, and the investigation of regulatory mechanisms and cell biological processes. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Bacterial Cell Surfaces: Methods and Protocols is aimed at the microbiologist, biochemist, or cell biologist, whether a beginning graduate student or a veteran experimentalist, who wishes to learn new methodologies and take advantage of the years of research and protocol optimization from the best laboratories around the world. | 1. A Bird's Eye View of the Bacterial LandscapeNatividad Ruiz2. Visualizing the Bacterial Cell Surface: An OverviewHarald Engelhardt3. Purification of the Outer Membrane Usher Protein and Periplasmic Chaperone-Subunit Complexes from the P and Type 1 Pilus SystemsNadine S. Henderson and David G. Thanassi4. Experimental Manipulation of the Microbial Functional Amyloid Called CurliYizhou Zhou, Daniel R. Smith, David A. Hufnagel, and Matthew R. Chapman5. Visualization of Gram-Positive Bacterial PiliChungyu Chang, I-Hsiu Huang, Antoni P. Hendrickx, and Hung Ton-That6. Single Cell Microfluidic Studies of Bacterial MotilityAdrien Ducret, Olivier Théodoly, and Tâm Mignot7. Chromatographic Analysis of the Escherichia coli Polysialic Acid CapsuleSusan M. Steenbergen and Eric R. Vimr8. Analysis of Exopolysaccharides in Myxococcus xanthus Using Confocal Laser Scanning MicroscopyWei Hu, Renate Lux, and Wenyuan Shi9. Assessment of Multidrug Efflux Assemblies by Surface Plasmon ResonanceElena B. Tikhonova and Helen I. Zgurskaya10. Fluorescence Microscopy and Proteomics to Investigate Subcellular Localization, Assembly, and Function of the Type II Secretion SystemTanya L. Johnson, Aleksandra E. Sikora, Ryszard A. Zielke, and Maria Sandkvist11. Pore Formation by T3SS Translocators: Liposome Leakage AssayEric Faudry, Caroline Perdu, and Ina Attrée12. Isolation of Bacterial Type IV Machine SubassembliesMayukh K. Sarkar, Seyyed I. Husnain, Simon J. Jakubowski, and Peter J. Christie13. Production and Crystallization of Bacterial Type V Secretion ProteinsHye-Jeong Yeo14. Assembly of Bacterial Outer Membrane ProteinsJan Grijpstra, Martine P. Bos, and Jan Tommassen15. The Outer Membrane of Gram-Negative Bacteria: Lipid A Isolation and CharacterizationJessica V. Hankins, James A. Madsen, Brittany D. Needham, Jennifer S. Brodbelt, and M. Stephen Trent16. Quantitative and Qualitative Preparations of Bacterial Outer Membrane VesiclesHalima Chutkan, Ian MacDonald, Andrew Manning, and Meta J. Kuehn17. In Vitro Peptidoglycan Synthesis Assay with Lipid II SubstrateJacob Biboy, Nhat Khai Bui, and Wa. Bookseller Inventory # K9781627032445

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