Update on Production of Recombinant Therapeutic Protein: Transient Gene Expression

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9781847359766: Update on Production of Recombinant Therapeutic Protein: Transient Gene Expression
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Over the past decade, the transient gene expression (TGE) technology platform has been actively pursued to produce a wide range of therapeutic proteins, monoclonal antibodies, and vaccines for mainly preclinical assessment, due to its short development times and low overall cost.

This book updates the latest advances in the field, with focusing on systematic description of the technology from cell lines, cell culture conditions, vector construction, expression strategy, current protocols, optimisation of the procedure, and potential for clinical application. As a conclusion, the author foresees that therapeutic biopharmaceutics will be manufactured for clinical development using TGE technology in the near future because of its fast development time, good protein expression, acceptable quality of product and due to the progress which has been made in analytical methodology and process quality control.

The objectives of this book are to summarise current TGE protocols, to describe optimisation of the technology through the latest advances, and to explore clinical applications of the technology. It gives the reader a good insight into the latest development and future application of the technology platform, including:

  • The current protocols from small to large scale for different cells.
  • Optimisation methods in construction designing, transfection procedures, and cell culture conditions.
  • Overall quality of the product from the transient gene expression.
  • Future clinical application of the technology platform.

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