Cryopreservation of Hematopoietic stem cells: Comparison of Cryopreservation of Hematopoietic stem cells using conventional freezing medium and Stemline Medium from SIGMA Aldrich

 
9783639287004: Cryopreservation of Hematopoietic stem cells: Comparison of Cryopreservation of Hematopoietic stem cells using conventional freezing medium and Stemline Medium from SIGMA Aldrich
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Cell populations residing in waste tissues (umbilical cord blood, and placenta) may be collected without any medical or ethical contraindications concerning the mother or newborn baby. Cord blood hematopoietic stem cells are routinely used for clinical transplants. The use of UCB for transplantation requires efficient cryopreservation procedures and revival methods. The purpose of this study was to compare the freezing of hematopoietic stem cells using 10% DMSO (conventional freezing medium i.e. Control) vs. Stemline medium from Sigma (Test). HSC recovery after freezing and thawing was assessed by viability testing, Evaluation of apoptosis using Annexin V- PI staining by fluorescence-activated cell sorting (FACS) analysis, and colony-forming ability under different storage periods. Apoptosis of cells occurs during cryopreservation as this is a stress phenomenon. Activity of Bcl-2 and Caspase-3 were also assessed using confocal imaging and mean fluorescence intensity was calculated.

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Nishant Mittal has finished his M.Tech in Industrial Biotechnology. Currently he is working as project Trainee at National Centre for Cell Science (NCCS), Pune, INDIA on Embryonic Stem Cells.

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Book Description Condition: New. Publisher/Verlag: VDM Verlag Dr. Müller | Comparison of Cryopreservation of Hematopoietic stem cells using conventional freezing medium and Stemline Medium from SIGMA Aldrich | Cell populations residing in waste tissues (umbilical cord blood, and placenta) may be collected without any medical or ethical contraindications concerning the mother or newborn baby. Cord blood hematopoietic stem cells are routinely used for clinical transplants. The use of UCB for transplantation requires efficient cryopreservation procedures and revival methods. The purpose of this study was to compare the freezing of hematopoietic stem cells using 10% DMSO (conventional freezing medium i.e. Control) vs. Stemline medium from Sigma (Test). HSC recovery after freezing and thawing was assessed by viability testing, Evaluation of apoptosis using Annexin V- PI staining by fluorescence-activated cell sorting (FACS) analysis, and colony-forming ability under different storage periods. Apoptosis of cells occurs during cryopreservation as this is a stress phenomenon. Activity of Bcl-2 and Caspase-3 were also assessed using confocal imaging and mean fluorescence intensity was calculated. | Format: Paperback | Language/Sprache: english | 72 pp. Seller Inventory # K9783639287004

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Book Description VDM Verlag Sep 2010, 2010. Taschenbuch. Condition: Neu. Neuware - Cell populations residing in waste tissues (umbilical cord blood, and placenta) may be collected without any medical or ethical contraindications concerning the mother or newborn baby. Cord blood hematopoietic stem cells are routinely used for clinical transplants. The use of UCB for transplantation requires efficient cryopreservation procedures and revival methods. The purpose of this study was to compare the freezing of hematopoietic stem cells using 10% DMSO (conventional freezing medium i.e. Control) vs. Stemline medium from Sigma (Test). HSC recovery after freezing and thawing was assessed by viability testing, Evaluation of apoptosis using Annexin V- PI staining by fluorescence-activated cell sorting (FACS) analysis, and colony-forming ability under different storage periods. Apoptosis of cells occurs during cryopreservation as this is a stress phenomenon. Activity of Bcl-2 and Caspase-3 were also assessed using confocal imaging and mean fluorescence intensity was calculated. 72 pp. Englisch. Seller Inventory # 9783639287004

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Book Description VDM Verlag Sep 2010, 2010. Taschenbuch. Condition: Neu. Neuware - Cell populations residing in waste tissues (umbilical cord blood, and placenta) may be collected without any medical or ethical contraindications concerning the mother or newborn baby. Cord blood hematopoietic stem cells are routinely used for clinical transplants. The use of UCB for transplantation requires efficient cryopreservation procedures and revival methods. The purpose of this study was to compare the freezing of hematopoietic stem cells using 10% DMSO (conventional freezing medium i.e. Control) vs. Stemline medium from Sigma (Test). HSC recovery after freezing and thawing was assessed by viability testing, Evaluation of apoptosis using Annexin V- PI staining by fluorescence-activated cell sorting (FACS) analysis, and colony-forming ability under different storage periods. Apoptosis of cells occurs during cryopreservation as this is a stress phenomenon. Activity of Bcl-2 and Caspase-3 were also assessed using confocal imaging and mean fluorescence intensity was calculated. 72 pp. Englisch. Seller Inventory # 9783639287004

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