Production of secondary metabolites from PLBs of Dendrobium candidum: The Standardization of Culture Condition for Efficient Induction of Secondary Metabolites

 
9783659293771: Production of secondary metabolites from PLBs of Dendrobium candidum: The Standardization of Culture Condition for Efficient Induction of Secondary Metabolites
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To establish an efficient culture protocol for production of biomass and bioactive compound, protocorm-like bodies (PLBs) of Dendrobium candidum were induced from stem segments using solid MS medium supplemented with 0.5 mg/L kinetin, 30g/L sucrose and 2.3 g/L Gelrite. By inoculating 80 g/L of PLBs in 1/2 MS medium containing 30 g/L sucrose at an initial medium pH of 5.0 under a 10 µmol m-2 s-1 PPF, the maximum growth as well as the maximum polysaccharide production was achieved. To elucidate optimum growth period, Dendrobium candidum PLBs were cultured for 49 days. Maximum PLBs growth was noted after 35 days of culture, however the polysaccharide content reached maximum after 21 days of culture. As an enhancement strategy medium replenishment and elicitation techniques were adopted. The highest accumulation of biomass and polysaccharide was achieved when culture fed with 1/2 MS medium containing 3% sucrose after 3 weeks. In addition, the highest accumulation of phenolics, flavonoids and total polysaccharides was obtained when cultures elicited with 100μM methyl jasmonate (MJ). Contrary, the higher concentration of MJ strongly inhibited the growth of PLBs.

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Dr. Md. Abdullahil Baque was born on 1 Feb. 1977 in Nilphamary Dist., Bangladesh. Currently he is an Assoc. Prof. at Sher-e-Bangla Agril. University, Dhaka, Bangladesh. Dr. Baque received his B. Sc. Ag. (Hons) from BAU, MS in Agronomy from BSMRAU, Bangladesh, Ph.D (Hort) from CBNU and also worked as a post-doctoral rerearcher at CBNU, south Korea.

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Book Description Condition: New. Publisher/Verlag: LAP Lambert Academic Publishing | The Standardization of Culture Condition for Efficient Induction of Secondary Metabolites | To establish an efficient culture protocol for production of biomass and bioactive compound, protocorm-like bodies (PLBs) of Dendrobium candidum were induced from stem segments using solid MS medium supplemented with 0.5 mg/L kinetin, 30g/L sucrose and 2.3 g/L Gelrite. By inoculating 80 g/L of PLBs in 1/2 MS medium containing 30 g/L sucrose at an initial medium pH of 5.0 under a 10 µmol m-2 s-1 PPF, the maximum growth as well as the maximum polysaccharide production was achieved. To elucidate optimum growth period, Dendrobium candidum PLBs were cultured for 49 days. Maximum PLBs growth was noted after 35 days of culture, however the polysaccharide content reached maximum after 21 days of culture. As an enhancement strategy medium replenishment and elicitation techniques were adopted. The highest accumulation of biomass and polysaccharide was achieved when culture fed with 1/2 MS medium containing 3% sucrose after 3 weeks. In addition, the highest accumulation of phenolics, flavonoids and total polysaccharides was obtained when cultures elicited with 100 M methyl jasmonate (MJ). Contrary, the higher concentration of MJ strongly inhibited the growth of PLBs. | Format: Paperback | Language/Sprache: english | 96 pp. Seller Inventory # K9783659293771

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Book Description LAP Lambert Academic Publishing Nov 2012, 2012. Taschenbuch. Condition: Neu. Neuware - To establish an efficient culture protocol for production of biomass and bioactive compound, protocorm-like bodies (PLBs) of Dendrobium candidum were induced from stem segments using solid MS medium supplemented with 0.5 mg/L kinetin, 30g/L sucrose and 2.3 g/L Gelrite. By inoculating 80 g/L of PLBs in 1/2 MS medium containing 30 g/L sucrose at an initial medium pH of 5.0 under a 10 µmol m-2 s-1 PPF, the maximum growth as well as the maximum polysaccharide production was achieved. To elucidate optimum growth period, Dendrobium candidum PLBs were cultured for 49 days. Maximum PLBs growth was noted after 35 days of culture, however the polysaccharide content reached maximum after 21 days of culture. As an enhancement strategy medium replenishment and elicitation techniques were adopted. The highest accumulation of biomass and polysaccharide was achieved when culture fed with 1/2 MS medium containing 3% sucrose after 3 weeks. In addition, the highest accumulation of phenolics, flavonoids and total polysaccharides was obtained when cultures elicited with 100 M methyl jasmonate (MJ). Contrary, the higher concentration of MJ strongly inhibited the growth of PLBs. 96 pp. Englisch. Seller Inventory # 9783659293771

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Md. Abdullahil Baque
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Book Description LAP Lambert Academic Publishing Nov 2012, 2012. Taschenbuch. Condition: Neu. Neuware - To establish an efficient culture protocol for production of biomass and bioactive compound, protocorm-like bodies (PLBs) of Dendrobium candidum were induced from stem segments using solid MS medium supplemented with 0.5 mg/L kinetin, 30g/L sucrose and 2.3 g/L Gelrite. By inoculating 80 g/L of PLBs in 1/2 MS medium containing 30 g/L sucrose at an initial medium pH of 5.0 under a 10 µmol m-2 s-1 PPF, the maximum growth as well as the maximum polysaccharide production was achieved. To elucidate optimum growth period, Dendrobium candidum PLBs were cultured for 49 days. Maximum PLBs growth was noted after 35 days of culture, however the polysaccharide content reached maximum after 21 days of culture. As an enhancement strategy medium replenishment and elicitation techniques were adopted. The highest accumulation of biomass and polysaccharide was achieved when culture fed with 1/2 MS medium containing 3% sucrose after 3 weeks. In addition, the highest accumulation of phenolics, flavonoids and total polysaccharides was obtained when cultures elicited with 100 M methyl jasmonate (MJ). Contrary, the higher concentration of MJ strongly inhibited the growth of PLBs. 96 pp. Englisch. Seller Inventory # 9783659293771

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Md. Humayun Kabir Shiragi
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Book Description LAP Lambert Academic Publishing. Paperback. Condition: New. 96 pages. Dimensions: 8.7in. x 5.9in. x 0.2in.To establish an efficient culture protocol for production of biomass and bioactive compound, protocorm-like bodies (PLBs) of Dendrobium candidum were induced from stem segments using solid MS medium supplemented with 0. 5 mgL kinetin, 30gL sucrose and 2. 3 gL Gelrite. By inoculating 80 gL of PLBs in 12 MS medium containing 30 gL sucrose at an initial medium pH of 5. 0 under a 10 mol m-2 s-1 PPF, the maximum growth as well as the maximum polysaccharide production was achieved. To elucidate optimum growth period, Dendrobium candidum PLBs were cultured for 49 days. Maximum PLBs growth was noted after 35 days of culture, however the polysaccharide content reached maximum after 21 days of culture. As an enhancement strategy medium replenishment and elicitation techniques were adopted. The highest accumulation of biomass and polysaccharide was achieved when culture fed with 12 MS medium containing 3 sucrose after 3 weeks. In addition, the highest accumulation of phenolics, flavonoids and total polysaccharides was obtained when cultures elicited with 100M methyl jasmonate (MJ). Contrary, the higher concentration of MJ strongly inhibited the growth of PLBs. This item ships from multiple locations. Your book may arrive from Roseburg,OR, La Vergne,TN. Paperback. Seller Inventory # 9783659293771

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Zhiwei Lu, Baque MD Abdullahil, Shiragi MD Humayun Kabir
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Book Description LAP Lambert Academic Publishing, United States, 2012. Paperback. Condition: New. Language: English. Brand new Book. To establish an efficient culture protocol for production of biomass and bioactive compound, protocorm-like bodies (PLBs) of Dendrobium candidum were induced from stem segments using solid MS medium supplemented with 0.5 mg/L kinetin, 30g/L sucrose and 2.3 g/L Gelrite. By inoculating 80 g/L of PLBs in 1/2 MS medium containing 30 g/L sucrose at an initial medium pH of 5.0 under a 10 mol m-2 s-1 PPF, the maximum growth as well as the maximum polysaccharide production was achieved. To elucidate optimum growth period, Dendrobium candidum PLBs were cultured for 49 days. Maximum PLBs growth was noted after 35 days of culture, however the polysaccharide content reached maximum after 21 days of culture. As an enhancement strategy medium replenishment and elicitation techniques were adopted. The highest accumulation of biomass and polysaccharide was achieved when culture fed with 1/2 MS medium containing 3% sucrose after 3 weeks. In addition, the highest accumulation of phenolics, flavonoids and total polysaccharides was obtained when cultures elicited with 100 M methyl jasmonate (MJ). Contrary, the higher concentration of MJ strongly inhibited the growth of PLBs. Seller Inventory # AAV9783659293771

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Md. Abdullahil Baque
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Book Description LAP Lambert Academic Publishing Nov 2012, 2012. Taschenbuch. Condition: Neu. This item is printed on demand - Print on Demand Neuware - To establish an efficient culture protocol for production of biomass and bioactive compound, protocorm-like bodies (PLBs) of Dendrobium candidum were induced from stem segments using solid MS medium supplemented with 0.5 mg/L kinetin, 30g/L sucrose and 2.3 g/L Gelrite. By inoculating 80 g/L of PLBs in 1/2 MS medium containing 30 g/L sucrose at an initial medium pH of 5.0 under a 10 µmol m-2 s-1 PPF, the maximum growth as well as the maximum polysaccharide production was achieved. To elucidate optimum growth period, Dendrobium candidum PLBs were cultured for 49 days. Maximum PLBs growth was noted after 35 days of culture, however the polysaccharide content reached maximum after 21 days of culture. As an enhancement strategy medium replenishment and elicitation techniques were adopted. The highest accumulation of biomass and polysaccharide was achieved when culture fed with 1/2 MS medium containing 3% sucrose after 3 weeks. In addition, the highest accumulation of phenolics, flavonoids and total polysaccharides was obtained when cultures elicited with 100 M methyl jasmonate (MJ). Contrary, the higher concentration of MJ strongly inhibited the growth of PLBs. 96 pp. Englisch. Seller Inventory # 9783659293771

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