Strategies for Production of Single Chain Antibody Fragment in E. coli: Production of Single chain antibody fragment

 
9783659482892: Strategies for Production of Single Chain Antibody Fragment in E. coli: Production of Single chain antibody fragment
View all copies of this ISBN edition:
 
 

Recombinant expression of antibody fragments in E. coli is often hampered by low expression, inefficient translocation and aggregation in the periplasm. Aggregation problem during in vitro-renaturation is also a limiting factor, when antibody fragments expressed in bacterial cytosol as inclusion bodies. The objective of this work was to achieve and optimise periplasmic production, and to explore novel strategies for in vitro renaturation from cytoplasmic IB’s. For the study, the single chain antibody fragment against the hapten oxazolone was chosen as the model system. In this book, results on periplasmic expression and expression as inclusion bodies and refolding thereof are presented. In expression in periplasmic effect of promoter strength and growth cultivation are presented. In vitro renaturation of IB's lead to establishment refolding protocols comprising of Ionic Liquids, which are emerging as green alternatives. Besides, we demonstrated usage of aromatic thiols as redox buffers components for protein refolding. Findings presented herein might be helpful for the of the periplasmic production of antibody fragments as as well as for establish protocols at physiological pH.

"synopsis" may belong to another edition of this title.

About the Author:

Ganesh Patil has completed his Ph.D from Martin Luther University, Halle, Germany. His expertise lies in the area of recombinant protein cloning, expression, purification, characterization. He is specialized in the area of process development on production of recombinant proteins, bacterial and viral antigens and monoclonal antibodies.

"About this title" may belong to another edition of this title.

Buy New View Book
List Price: US$ 68.00
US$ 52.37

Convert Currency

Shipping: US$ 8.40
From United Kingdom to U.S.A.

Destination, Rates & Speeds

Add to Basket

Top Search Results from the AbeBooks Marketplace

1.

Patil Ganesh
Published by LAP Lambert Academic Publishing 2014-02-06 (2014)
ISBN 10: 3659482897 ISBN 13: 9783659482892
New paperback Quantity Available: > 20
Seller:
Blackwell's
(Oxford, OX, United Kingdom)
Rating
[?]

Book Description LAP Lambert Academic Publishing 2014-02-06, 2014. paperback. Condition: New. Seller Inventory # 9783659482892

More information about this seller | Contact this seller

Buy New
US$ 52.37
Convert Currency

Add to Basket

Shipping: US$ 8.40
From United Kingdom to U.S.A.
Destination, Rates & Speeds

2.

Patil, Ganesh
ISBN 10: 3659482897 ISBN 13: 9783659482892
New Quantity Available: 1
Seller:
Rating
[?]

Book Description Condition: New. Publisher/Verlag: LAP Lambert Academic Publishing | Production of Single chain antibody fragment | Recombinant expression of antibody fragments in E. coli is often hampered by low expression, inefficient translocation and aggregation in the periplasm. Aggregation problem during in vitro-renaturation is also a limiting factor, when antibody fragments expressed in bacterial cytosol as inclusion bodies. The objective of this work was to achieve and optimise periplasmic production, and to explore novel strategies for in vitro renaturation from cytoplasmic IB s. For the study, the single chain antibody fragment against the hapten oxazolone was chosen as the model system. In this book, results on periplasmic expression and expression as inclusion bodies and refolding thereof are presented. In expression in periplasmic effect of promoter strength and growth cultivation are presented. In vitro renaturation of IB's lead to establishment refolding protocols comprising of Ionic Liquids, which are emerging as green alternatives. Besides, we demonstrated usage of aromatic thiols as redox buffers components for protein refolding. Findings presented herein might be helpful for the of the periplasmic production of antibody fragments as as well as for establish protocols at physiological pH. | Format: Paperback | Language/Sprache: english | 181 gr | 220x150x6 mm | 124 pp. Seller Inventory # K9783659482892

More information about this seller | Contact this seller

Buy New
US$ 57.56
Convert Currency

Add to Basket

Shipping: US$ 3.66
From Germany to U.S.A.
Destination, Rates & Speeds

3.

Patil, Ganesh
Published by LAP Lambert Academic Publishing (2014)
ISBN 10: 3659482897 ISBN 13: 9783659482892
New Quantity Available: > 20
Print on Demand
Seller:
Pbshop
(Wood Dale, IL, U.S.A.)
Rating
[?]

Book Description LAP Lambert Academic Publishing, 2014. PAP. Condition: New. New Book. Shipped from US within 10 to 14 business days. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. Seller Inventory # IQ-9783659482892

More information about this seller | Contact this seller

Buy New
US$ 64.57
Convert Currency

Add to Basket

Shipping: US$ 3.99
Within U.S.A.
Destination, Rates & Speeds

4.

Patil, Ganesh
Published by LAP Lambert Academic Publishing (2014)
ISBN 10: 3659482897 ISBN 13: 9783659482892
New Quantity Available: > 20
Print on Demand
Seller:
Books2Anywhere
(Fairford, GLOS, United Kingdom)
Rating
[?]

Book Description LAP Lambert Academic Publishing, 2014. PAP. Condition: New. New Book. Delivered from our UK warehouse in 4 to 14 business days. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. Seller Inventory # LQ-9783659482892

More information about this seller | Contact this seller

Buy New
US$ 57.94
Convert Currency

Add to Basket

Shipping: US$ 12.60
From United Kingdom to U.S.A.
Destination, Rates & Speeds

5.

Ganesh Patil
ISBN 10: 3659482897 ISBN 13: 9783659482892
New Quantity Available: 5
Seller:
ReadWhiz
(Portland, OR, U.S.A.)
Rating
[?]

Book Description Condition: New. Seller Inventory # ria9783659482892_ing

More information about this seller | Contact this seller

Buy New
US$ 82.52
Convert Currency

Add to Basket

Shipping: FREE
Within U.S.A.
Destination, Rates & Speeds

6.

Ganesh Patil
Published by LAP Lambert Academic Publishing Feb 2014 (2014)
ISBN 10: 3659482897 ISBN 13: 9783659482892
New Taschenbuch Quantity Available: 1
Seller:
Rheinberg-Buch
(Bergisch Gladbach, Germany)
Rating
[?]

Book Description LAP Lambert Academic Publishing Feb 2014, 2014. Taschenbuch. Condition: Neu. Neuware - Recombinant expression of antibody fragments in E. coli is often hampered by low expression, inefficient translocation and aggregation in the periplasm. Aggregation problem during in vitro-renaturation is also a limiting factor, when antibody fragments expressed in bacterial cytosol as inclusion bodies. The objective of this work was to achieve and optimise periplasmic production, and to explore novel strategies for in vitro renaturation from cytoplasmic IB s. For the study, the single chain antibody fragment against the hapten oxazolone was chosen as the model system. In this book, results on periplasmic expression and expression as inclusion bodies and refolding thereof are presented. In expression in periplasmic effect of promoter strength and growth cultivation are presented. In vitro renaturation of IB's lead to establishment refolding protocols comprising of Ionic Liquids, which are emerging as green alternatives. Besides, we demonstrated usage of aromatic thiols as redox buffers components for protein refolding. Findings presented herein might be helpful for the of the periplasmic production of antibody fragments as as well as for establish protocols at physiological pH. 124 pp. Englisch. Seller Inventory # 9783659482892

More information about this seller | Contact this seller

Buy New
US$ 62.90
Convert Currency

Add to Basket

Shipping: US$ 21.11
From Germany to U.S.A.
Destination, Rates & Speeds

7.

Ganesh Patil
Published by LAP Lambert Academic Publishing Feb 2014 (2014)
ISBN 10: 3659482897 ISBN 13: 9783659482892
New Taschenbuch Quantity Available: 1
Seller:
BuchWeltWeit Inh. Ludwig Meier e.K.
(Bergisch Gladbach, Germany)
Rating
[?]

Book Description LAP Lambert Academic Publishing Feb 2014, 2014. Taschenbuch. Condition: Neu. Neuware - Recombinant expression of antibody fragments in E. coli is often hampered by low expression, inefficient translocation and aggregation in the periplasm. Aggregation problem during in vitro-renaturation is also a limiting factor, when antibody fragments expressed in bacterial cytosol as inclusion bodies. The objective of this work was to achieve and optimise periplasmic production, and to explore novel strategies for in vitro renaturation from cytoplasmic IB s. For the study, the single chain antibody fragment against the hapten oxazolone was chosen as the model system. In this book, results on periplasmic expression and expression as inclusion bodies and refolding thereof are presented. In expression in periplasmic effect of promoter strength and growth cultivation are presented. In vitro renaturation of IB's lead to establishment refolding protocols comprising of Ionic Liquids, which are emerging as green alternatives. Besides, we demonstrated usage of aromatic thiols as redox buffers components for protein refolding. Findings presented herein might be helpful for the of the periplasmic production of antibody fragments as as well as for establish protocols at physiological pH. 124 pp. Englisch. Seller Inventory # 9783659482892

More information about this seller | Contact this seller

Buy New
US$ 62.90
Convert Currency

Add to Basket

Shipping: US$ 21.11
From Germany to U.S.A.
Destination, Rates & Speeds

8.

Ganesh Patil
Published by LAP LAMBERT Academic Publishing
ISBN 10: 3659482897 ISBN 13: 9783659482892
New Paperback Quantity Available: > 20
Seller:
BuySomeBooks
(Las Vegas, NV, U.S.A.)
Rating
[?]

Book Description LAP LAMBERT Academic Publishing. Paperback. Condition: New. 124 pages. Dimensions: 8.7in. x 5.9in. x 0.3in.Recombinant expression of antibody fragments in E. coli is often hampered by low expression, inefficient translocation and aggregation in the periplasm. Aggregation problem during in vitro-renaturation is also a limiting factor, when antibody fragments expressed in bacterial cytosol as inclusion bodies. The objective of this work was to achieve and optimise periplasmic production, and to explore novel strategies for in vitro renaturation from cytoplasmic IBs. For the study, the single chain antibody fragment against the hapten oxazolone was chosen as the model system. In this book, results on periplasmic expression and expression as inclusion bodies and refolding thereof are presented. In expression in periplasmic effect of promoter strength and growth cultivation are presented. In vitro renaturation of IBs lead to establishment refolding protocols comprising of Ionic Liquids, which are emerging as green alternatives. Besides, we demonstrated usage of aromatic thiols as redox buffers components for protein refolding. Findings presented herein might be helpful for the of the periplasmic production of antibody fragments as as well as for establish protocols at physiological pH. This item ships from multiple locations. Your book may arrive from Roseburg,OR, La Vergne,TN. Paperback. Seller Inventory # 9783659482892

More information about this seller | Contact this seller

Buy New
US$ 96.96
Convert Currency

Add to Basket

Shipping: FREE
Within U.S.A.
Destination, Rates & Speeds

9.

Patil Ganesh
Published by LAP Lambert Academic Publishing, United States (2014)
ISBN 10: 3659482897 ISBN 13: 9783659482892
New Paperback Quantity Available: 1
Seller:
The Book Depository EURO
(London, United Kingdom)
Rating
[?]

Book Description LAP Lambert Academic Publishing, United States, 2014. Paperback. Condition: New. Language: English . Brand New Book. Recombinant expression of antibody fragments in E. coli is often hampered by low expression, inefficient translocation and aggregation in the periplasm. Aggregation problem during in vitro-renaturation is also a limiting factor, when antibody fragments expressed in bacterial cytosol as inclusion bodies. The objective of this work was to achieve and optimise periplasmic production, and to explore novel strategies for in vitro renaturation from cytoplasmic IB s. For the study, the single chain antibody fragment against the hapten oxazolone was chosen as the model system. In this book, results on periplasmic expression and expression as inclusion bodies and refolding thereof are presented. In expression in periplasmic effect of promoter strength and growth cultivation are presented. In vitro renaturation of IB s lead to establishment refolding protocols comprising of Ionic Liquids, which are emerging as green alternatives. Besides, we demonstrated usage of aromatic thiols as redox buffers components for protein refolding. Findings presented herein might be helpful for the of the periplasmic production of antibody fragments as as well as for establish protocols at physiological pH. Seller Inventory # KNV9783659482892

More information about this seller | Contact this seller

Buy New
US$ 94.37
Convert Currency

Add to Basket

Shipping: US$ 4.19
From United Kingdom to U.S.A.
Destination, Rates & Speeds

10.

Ganesh Patil
Published by LAP Lambert Academic Publishing Feb 2014 (2014)
ISBN 10: 3659482897 ISBN 13: 9783659482892
New Taschenbuch Quantity Available: 1
Print on Demand
Seller:
AHA-BUCH GmbH
(Einbeck, Germany)
Rating
[?]

Book Description LAP Lambert Academic Publishing Feb 2014, 2014. Taschenbuch. Condition: Neu. This item is printed on demand - Print on Demand Neuware - Recombinant expression of antibody fragments in E. coli is often hampered by low expression, inefficient translocation and aggregation in the periplasm. Aggregation problem during in vitro-renaturation is also a limiting factor, when antibody fragments expressed in bacterial cytosol as inclusion bodies. The objective of this work was to achieve and optimise periplasmic production, and to explore novel strategies for in vitro renaturation from cytoplasmic IB s. For the study, the single chain antibody fragment against the hapten oxazolone was chosen as the model system. In this book, results on periplasmic expression and expression as inclusion bodies and refolding thereof are presented. In expression in periplasmic effect of promoter strength and growth cultivation are presented. In vitro renaturation of IB's lead to establishment refolding protocols comprising of Ionic Liquids, which are emerging as green alternatives. Besides, we demonstrated usage of aromatic thiols as redox buffers components for protein refolding. Findings presented herein might be helpful for the of the periplasmic production of antibody fragments as as well as for establish protocols at physiological pH. 124 pp. Englisch. Seller Inventory # 9783659482892

More information about this seller | Contact this seller

Buy New
US$ 62.90
Convert Currency

Add to Basket

Shipping: US$ 36.10
From Germany to U.S.A.
Destination, Rates & Speeds

There are more copies of this book

View all search results for this book