Chicken Egg Yolk Antibodies, Production and Application: IgY-Technology (Springer Lab Manuals)

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9783662044902: Chicken Egg Yolk Antibodies, Production and Application: IgY-Technology (Springer Lab Manuals)

Polyclonal antibodies, widely used in research and diagnostics, are conventionally isolated from the blood of immunised mammals. The fact that antibodies can also be detected in the yolk of eggs laid by immunised hens, led to the development of the yolk antibody technology as an alternative method less stressful to animals. Hens can be kept under nearly natural conditions and antibodies be isolated from the eggs.
Further, the IgY-technology has the advantage that avian antibodies can be produced against several antigens which do not induce a immune response in mammals. Protocols on how to keep and immunise hens and on the extraction, isolation and use of antibodies from yolk are described in detail in this manual.
For their work on yolk antibodies the authors received the FISEA (International Foundation for the Substitution of Animal Experimentation, Luxembourg) prize in 1997.

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Polyclonal antibodies, widely used in research and diagnostics, are conventionally isolated from the blood of immunised mammals, especially rabbits. The fact that antibodies can also be detected in the yolk of eggs laid by immunised hens, led to the development of the yolk antibody technology as an alternative method less stressful to animals. Since hens can be kept under nearly natural conditions and antibodies be isolated from the collected eggs, this technology has become an interesting alternative to the ,bloody" techniques. For their work on yolk antibodies, the authors of this book received the FISEA prize (International Foundation for the Substitution of Animal Experimentation, Luxembourg) in 1997. Protocols on how to keep and immunise hens and on the extraction, isolation and use of antibodies from yolk are described in detail in this manual. These practical instructions are complemented by a short introduction to the hen's humoral immune system and a section on the pros and cons of chicken yolk antibodies compared to those of mammals'serum. Avian antibodies have been shown to be in some applications even more effective than mamalian antibodies, especially when phylogenetically highly conserved antigens have been used for immunisation.

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Book Description Springer. Paperback. Book Condition: New. Paperback. 255 pages. Dimensions: 9.0in. x 6.0in. x 0.7in.Polyclonal antibodies, widely used in research and diagnostics, are conventionally isolated from the blood of immunised mammals. The fact that antibodies can also be detected in the yolk of eggs laid by immunised hens, led to the development of the yolk antibody technology as an alternative method less stressful to animals. Hens can be kept under nearly natural conditions and antibodies be isolated from the eggs. Further, the IgY-technology has the advantage that avian antibodies can be produced against several antigens which do not induce a immune response in mammals. Protocols on how to keep and immunise hens and on the extraction, isolation and use of antibodies from yolk are described in detail in this manual. For their work on yolk antibodies the authors received the FISEA (International Foundation for the Substitution of Animal Experimentation, Luxembourg) prize in 1997. This item ships from multiple locations. Your book may arrive from Roseburg,OR, La Vergne,TN. Paperback. Bookseller Inventory # 9783662044902

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Book Description Springer-Verlag Berlin and Heidelberg GmbH Co. KG, Germany, 2013. Paperback. Book Condition: New. 2001 ed.. Language: English . Brand New Book ***** Print on Demand *****.Polyclonal antibodies, widely used in research and diagnostics, are conventionally isolated from the blood of immunised mammals. The fact that antibodies can also be detected in the yolk of eggs laid by immunised hens, led to the development of the yolk antibody technology as an alternative method less stressful to animals. Hens can be kept under nearly natural conditions and antibodies be isolated from the eggs. Further, the IgY-technology has the advantage that avian antibodies can be produced against several antigens which do not induce a immune response in mammals. Protocols on how to keep and immunise hens and on the extraction, isolation and use of antibodies from yolk are described in detail in this manual. For their work on yolk antibodies the authors received the FISEA (International Foundation for the Substitution of Animal Experimentation, Luxembourg) prize in 1997. Bookseller Inventory # AAV9783662044902

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