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This is a Ph.D. dissertation. Contents include: Introduction: The normal salivary gland, Salivary gland tumors, Pleomorphic adenoma of the salivary glands, PLAG1, Unraveling human cancer using the mouse as a model system, Salivary gland transgenesis; Aims and Rationale; Material and Methods: Immunohistochemistry, Cytogenetic analysis, FISH analysis, Immunoflourescence, Cloning strategies, DNA sequencing, Transfections and luciferase assay, Western blot analysis, Generation of transgenic mouse strain by zygote micro-injection, PCR analysis, Isolation of RNA, Northern blot analysis, Isolation of DNA, Southern blot analysis, Labeling of DNA probes, Histology, Gene expression profiling; Results: Immunohistological localization studies of PLAG1 in human pleomorphic adenoma of the salivary gland, Human PLAG1 transgenic mouse strain, Study of PLAG1 and PLAGL2 target genes by microarray analysis; Discussion and Perspectives: Immunohistological localization studies of PLAG1 in the human pleomorphic adenoma of the salivary gland, Human PLAG1 transgenic mouse strain, Study of PLAG1 and PLAGL2 target genes by microarray analysis; General conclusions; Summary; References.
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