Cloning and Expression of Recombinant Fasciola Antigen
Available from more sellers
Paperback. 120 pages. Dimensions: 8.7in. x 5.9in. x 0.3in.Fasciolosis is a liver disease caused by Fasciola hepatica or F. gigantica that causes significant economic loss in cattles, other animal species and man. The appearance of Fasciola hepatica populations that are resistant to common flukicidal drugs make a need for the development of anti-liver fluke vaccines. Though we selected a target Fasciola gene that expresses (rFhp) protein, in which its native form shows significant induction to lymphoproliferative response of Peripheral blood mononuclear cells. Then we amplified the gene through Polymerase chain reaction process using gt11 specific primers. This gene was cloned through TOPO (TA) cloning vector in XL10-Gold competent cells, then positive colonies that contain rTOPO were used for the excision of the gene to be expressed in expression vectors. ECORI digestions of the purified plasmids were done to detect the existence of the target insert. The results showed the excision of Fh400 from recombinant PQE32 vector and its size determined at 500bp. Expression and screening of small cultures was done and a 6xHis-tagged protein was purified and stained on SDS-PAGE that appeared at about 14. 5 kDa. This item ships from multiple locations. Your book may arrive from Roseburg,OR, La Vergne,TN. Bookseller Inventory # 9783659147913
About this title:
About the Author: Dr. Nahed Ahmed Hussien, I am a Lecturer at Cairo Univ., Faculty of science, Zoology ?Department from 2010. Contributed at the Egyptian Reference Diagnostic ?Center in Fasciola Vaccine projects, and also in Welcome Trust HCV Projects. My ?work experience was mainly on Molecular Biology branch including DNA ?extraction, PCR, SSCP & Comet assay.
Title: Cloning and Expression of Recombinant ...
Publisher: LAP LAMBERT Academic Publishing
Book Condition: New
Book Type: Paperback
Payment Methods Accepted by Seller
We guarantee the condition of every book as it's described on the Abebooks web
sites. If you're dissatisfied with your purchase (Incorrect Book/Not as
Described/Damaged) or if the order hasn't arrived, you're eligible for a refund
within 30 days of the estimated delivery date. If you've changed your mind about a book that you've ordered, please use the Ask bookseller a question link to contact us and we'll respond within 2 business days.
BuySomeBooks is operated by Drive-On-In, Inc., a Nevada co...[More Information]
Orders usually ship within 1-2 business days. Books are shipped from multiple locations so your order may arrive from Las Vegas,NV, Roseburg,OR, La Vergne,TN, Momence,IL, or Commerce,GA.
Store Description: BuySomeBooks is great place to get your books online. With over eight million titles available we're sure to have what you're looking for. Despite having a large selection of new books available for immediate shipment and excellent customer service, people still tell us they prefer us because of our prices.