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Manual of Microbiology: Tools & Techniques, Second Edition

Kanika Sharma

ISBN 10: 8180521435 / ISBN 13: 9788180521430
Published by Ane Books Pvt. Ltd, 2011
New Condition: New Soft cover
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Manual of Microbiology Tools & Techniques is a complete text with an Integrated approach. It will be of use for graduate and post graduate students of Biological Sciences, Microbiology, Biotechnology and allied branches. A Student friendly manual, written essentially to develop the skills in practical techniques of microbiology. It includes variety of Experiments needed to assist Teaching of the subject. Each experiment contains precise information of the principle, purpose and precautions to be taken. Easy to follow Pattern of Methodology defines each step accurately. The Book will also prove useful to Teachers and people involved in microbial Research as it includes information about equipments, laboratory setup, list of relevant institutes and laboratories, literature available etc. Contents I. INTRODUCTION TO Microbiology : 1. General Characteristics of Microorganisms 2. Classification of Microorganisms 3. Nomenclature of Microorganisms 4. Major Groups of Prokaryotes II. TOOLS OF MICROBIOLOGY : 1. Microscopes 2. History of Development of Microscopes 3. Principles of Microscopy 4. Care and Use of the Microscope 5. Spectrophotometer 6. pH Meter 7. Micrometer 8. Hemocytometer 9. Autoclave 10. Centrifuge 11. Balance 12. Pipettes 13. Vacuum Filtration 14. Water Bath 15. Biological Safety Cabinets 16. Glassware 17. Inoculation Needle and Loop 18. Incubator 19. Colony Counter 20. Lyophilizer (Freeze Dryer) 21. Micro-well Plates III. TECHNIQUES OF MICROBIOLOGY : 1. Sterilisation 2. Observation of Microorganisms 3. Simple Staining 4. Negative Staining 5. Differential Staining 6. Micro Chemical Staining 7. Cytological Staining 8. Some other Staining Techniques 9. Culture Techniques 10. Anaerobic Culture 11. Development of Pure Culture 12. Microscopic Measurements 13. Sample Collection 14. Titration IV. CULTURE MEDIA : 1. Components of Media 2. Role of Substances Added to the Media 3. Types of Media 4. Interaction of Media Components 5. Preparation of Nutrient Medium V. ISOLATION, PURIFICATION AND Identification OF Bacteria : 1. Isolation and Purification of Bacteria 2. Identification of Bacteria 3. Rapid Methods for Identifying Microbes VI. MAINTENANCE AND Storage OF BACTERIAL STRAINS VII. LABORATORY EXERCISES TO Study BACTERIA : 1. Study of Motility by Hanging Drop Method 2. Calibration of Micrometer and Measurement of Microbial Size 3. Simple Staining of Bacteria 4. Negative Staining of Bacteria 5. Differential Staining : Gram Staining 6. Differential Staining : Acid Fast Staining 7. Cytological Staining : Endospore 8. Cytological Staining : Capsule 9. Cytological Staining : Flagella 10. Staining of intracellular Metabolites 11. Culture of Bacteria on Solid Medium 12. Culture of Bacteria in Liquid Medium (Broth Culture) 13. Isolation and Development of Pure Cultures of Bacteria 14. Determination of Cell Numbers by Turbidimetric Method 15. Determination of Cell Numbers by Hemacytometer 16. Determination of Viable Count 17. Anaerobic Culture of Bacteria 18. Sampling of Air for Presence of Microbes 19. Culture of Bacteria to Show Ubiquitous Presence of Microorganisms 20. Isolation of Bacteria by Using Differential and Selective Media 21. Enrichment Culture of Pseudomonas VIII. STUDY OF BACTERIAL GROWTH : 1. Enumeration of Cell Numbers 2. Determination of Viable Count 3. To Prepare the Growth Curve of Bacteria 4. To Compare Growth of Bacteria on Different Media 19. STUDY OF Environmental INFLUENCES ON BACTERIAL GROWTH : 1. Factors That Effect Growth 2. Study of Effect of Temperature on Growth 3. Study of Lethal Effect of Temperature on Growth 4. Study of Heat Resistance of Vegetative Cells and Spores 5. Study of Effect of Osmotic Pressure on Growth 6. Study of Effect of Medium pH on Growth 7. Study of Energy Source Preference on Growth 8. Study of Effect of Energy Source and Role of Buffers on Growth 9. Printed Pages: 424. Bookseller Inventory # 44405

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Bibliographic Details

Title: Manual of Microbiology: Tools & Techniques, ...

Publisher: Ane Books Pvt. Ltd

Publication Date: 2011

Binding: Softcover

Book Condition: New

Edition: 5th or later edition.

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