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Molecular Study of Aeromonas in North East India

Indu Sharma

ISBN 10: 3639283775 / ISBN 13: 9783639283778
Published by VDM Verlag
New Condition: New Soft cover
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156 pages. Dimensions: 8.7in. x 5.9in. x 0.4in.Molecular typing of Aeromonas isolates by multiplex PCR assay exhibited various sizes of amplification products as predicted from the design of the primers. The assay used for the molecular typing of all the 38 isolates revealed that all the isolates belonged to Aeromonas spp. The alkali-lyses method of plasmid DNA isolation resulted in high yield of plasmids. The RAPD revealed that amplicon sizes ranged from 250 bp to 2. 9 Kb with a common 250 bp fragments in all the isolates and 75 similarity was observed in all the representative isolates. Results of mouse paw oedema test indicated that 21 isolates out 38 tested were positive in this test. The relative thickness for enterotoxicity was observed to be higher than 121. 0 3. 8. This item ships from multiple locations. Your book may arrive from Roseburg,OR, La Vergne,TN. Bookseller Inventory # 9783639283778

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Bibliographic Details

Title: Molecular Study of Aeromonas in North East ...

Publisher: VDM Verlag

Binding: Paperback

Book Condition:New

Book Type: Paperback

About this title

Synopsis:

Molecular typing of Aeromonas isolates by multiplex PCR assay exhibited various sizes of amplification products as predicted from the design of the primers. The assay used for the molecular typing of all the 38 isolates revealed that all the isolates belonged to Aeromonas spp.The alkali-lyses method of plasmid DNA isolation resulted in high yield of plasmids.The RAPD revealed that amplicon sizes ranged from 250 bp to 2.9 Kb with a common >250 bp fragments in all the isolates and 75% similarity was observed in all the representative isolates.Results of mouse paw oedema test indicated that 21 isolates out 38 tested were positive in this test. The relative thickness for enterotoxicity was observed to be higher than 121.0 ± 3.8.

About the Author:

Dr. Indu Sharma has specialized in Veterinary public health and is serving currently in Assam University, Silchar as Assistant Professor. Her research work includes ?Bacterial profile of E. coli and Salmonella in goat, pig and poultry meat and its public health significance'.

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