Practical Flow Cytometry in Haematology: 100 Worked Examples

Mike Leach

ISBN 10: 1118747038 / ISBN 13: 9781118747032
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The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis.

This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors’ institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis.

The reader will review May−Grünwald−Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference.

Practical Flow Cytometry in Haematology: 100 Worked Examples:

  • Provides a practical, example-based resource for flow cytometry
  • Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care
  • Includes both malignant and benign conditions
  • Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207)

Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.

About the Author:

Mike Leach FRCP, FRCPath
Consultant Haematologist and Honorary Senior Lecturer, Haematology Laboratories and West of Scotland Cancer Centre Gartnavel General Hospital, Glasgow, UK

Mark Drummond PhD, FRCPath Consultant Haematologist and Honorary Senior Lecturer, Haematology Laboratories and West of Scotland Cancer Centre Gartnavel General Hospital, Glasgow, UK

Allyson Doig MSc, FIBMS Haemato-Oncology Laboratory Manager, Haematology Laboratories Gartnavel General Hospital, Glasgow, UK

Pam McKay FRCP FRCPath Consultant Haematologist, Haematology Laboratories and West of Scotland Cancer Centre Gartnavel General Hospital, Glasgow, UK

Bob Jackson MB MRCPI FRCPath Haematopathology Laboratories, Southern General Hospital, Glasgow, UK

Barbara J. Bain MB BS, FRACP, FRCPath, Professor of Diagnostic Haematology, St Mary’s Hospital Campus of Imperial College Faculty of Medicine, London and Honorary Consultant Haematologist, St Mary’s Hospital, London, UK

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Mike;Drummond Leach
Published by John Wiley & Sons (2015)
ISBN 10: 1118747038 ISBN 13: 9781118747032
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Book Description John Wiley & Sons, 2015. Gebundene Ausgabe. Book Condition: Gebraucht. Gebraucht - Gut - Dieses Begleitbuch zu Practical Flow Cytometry in Haematology Diagnosis enthält 100 Beispiele klinischer Fälle, aus dem Alltag des Autors. Die einzelnen Fallbeispiele werden durch Daten aus den Bereichen periphäre Blut- und Knochenmarkzytologie, Gewebepathologie, Zytogenetik und Molekulardiagnostik ergänzt, die im Einzelfall eine Diagnose anhand der Tumorklassifikation der WHO bei hämotopoetischem und lymphoidem Gewebe ermöglichen. - Referenzwerk zur Durchflusszytometrie, das die Methode anhand von Beispielen aus der Praxis erläutert. - Bietet Übungen zur Überprüfung des Lernstatus und bereitet auf das FRCPath-Examen vor. - Zeigt, wie Ergebnisse der Durchflusszytometrie interpretiert und anwendet werden, um die Behandlung der Patienten zu unterstützen. - Behandelt sowohl krankhafte als auch normale Befunde. - Begleitbuch zu Practical Flow Cytometry in Haematology Diagnosis des Autors (ISBN 978-0-470-67120-7). A companion book to Practical Flow Cytometry in Haematology Diagnosis . This title contains 100 worked examples drawn from real clinical cases presenting to the authors' institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. Provides a practical, example-based resource for flow cytometry Lets the reader assess their knowledge Assists the reader with study for the FRCPath in haematology Demonstrates how flow cytometric results should be interpreted and applied to optimize patient care Covers both malignant and benign conditions Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis , by the same author (ISBN 978-0-470-67120-7) 416 pp. Englisch. Bookseller Inventory # INF3002995060

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Book Description John Wiley & Sons, 2015. Gebundene Ausgabe. Book Condition: Gebraucht. Gebraucht - Sehr gut - Dieses Begleitbuch zu Practical Flow Cytometry in Haematology Diagnosis enthält 100 Beispiele klinischer Fälle, aus dem Alltag des Autors. Die einzelnen Fallbeispiele werden durch Daten aus den Bereichen periphäre Blut- und Knochenmarkzytologie, Gewebepathologie, Zytogenetik und Molekulardiagnostik ergänzt, die im Einzelfall eine Diagnose anhand der Tumorklassifikation der WHO bei hämotopoetischem und lymphoidem Gewebe ermöglichen. - Referenzwerk zur Durchflusszytometrie, das die Methode anhand von Beispielen aus der Praxis erläutert. - Bietet Übungen zur Überprüfung des Lernstatus und bereitet auf das FRCPath-Examen vor. - Zeigt, wie Ergebnisse der Durchflusszytometrie interpretiert und anwendet werden, um die Behandlung der Patienten zu unterstützen. - Behandelt sowohl krankhafte als auch normale Befunde. - Begleitbuch zu Practical Flow Cytometry in Haematology Diagnosis des Autors (ISBN 978-0-470-67120-7). A companion book to Practical Flow Cytometry in Haematology Diagnosis . This title contains 100 worked examples drawn from real clinical cases presenting to the authors' institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. Provides a practical, example-based resource for flow cytometry Lets the reader assess their knowledge Assists the reader with study for the FRCPath in haematology Demonstrates how flow cytometric results should be interpreted and applied to optimize patient care Covers both malignant and benign conditions Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis , by the same author (ISBN 978-0-470-67120-7) 416 pp. Englisch. Bookseller Inventory # INF3003006453

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Book Description John Wiley and Sons Ltd, United Kingdom, 2015. Hardback. Book Condition: New. 1. Auflage. Language: English . Brand New Book. The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis. The reader will review May Grunwald Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (HE)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference. Practical Flow Cytometry in Haematology: 100 Worked Examples: * Provides a practical, example-based resource for flow cytometry * Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care * Includes both malignant and benign conditions * Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207) Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations. Bookseller Inventory # AAH9781118747032

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Book Description John Wiley and Sons Ltd, United Kingdom, 2015. Hardback. Book Condition: New. 1. Auflage. Language: English . Brand New Book. The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis. The reader will review May Grunwald Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (HE)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference. Practical Flow Cytometry in Haematology: 100 Worked Examples: * Provides a practical, example-based resource for flow cytometry * Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care * Includes both malignant and benign conditions * Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207) Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations. Bookseller Inventory # AAH9781118747032

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