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Practical Histochemistry (Wiley-Interscience Publication)

Chayen, Joseph, etc.

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ISBN 10: 0471149500 / ISBN 13: 9780471149507
Published by John Wiley & Sons Ltd, 1973
Used Condition: Good
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Ships from Reno, NV. Shows some signs of wear, and may have some markings on the inside. Bookseller Inventory # GRP90054885

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Bibliographic Details

Title: Practical Histochemistry (Wiley-Interscience...

Publisher: John Wiley & Sons Ltd

Publication Date: 1973

Book Condition:Good

Edition: Revised.

About this title


Practical Histochemistry Second Edition J. Chayen and L. Bitensky Unit of Cellular Pharmacology and Toxicology, Robens Institute, University of Surrey, UK Whereas in the past histochemistry has been primarily a way of colouring cells and tissue sections, over the last fifteen years it has evolved into a precise and quantitative form of microbiochemistry. New techniques and reagents have been developed which now form the basis of such diverse procedures as highly sensitive bioassays of polypeptides and the monitoring of open heart surgery and heart transplantation. Cell function is left unimpaired by ‘hidden reagents’ which do not release their active moieties until triggered by the products of biochemical reactions, and new methods prevent the loss of soluble active groups from cells and tissues even during prolonged reaction times. The second edition of this highly successful book has been extensively revised and updated to give an authoritative and practical account of the techniques of traditional and modern histochemistry. It conveys the sense of excitement that has been generated in developing a rigorous biochemistry that can be performed at the cellular level within a tissue.

From the Publisher:

Offers students and researchers a comprehensive guide to practical and reliable applications of this precise, quantitative branch of micro-biochemistry. Includes methods for keeping all components inside the cells or sections during prolonged reaction times; new ``hidden capture'' reactions that do not allow potentially inhibitory moieties to come into contact with cells or tissue are also discussed; quantification so that activites per cell correspond to the activites normally measurable biochemically; and the use of quantitative polarized light microscopy for measuring the alignment of significant molecules.

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