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Receptor Purification Volume 1 Receptors for CNS Agents, Growth Factors, Hormones, and Related Substances

Gerald Litwack

Published by Humana Press
ISBN 10: 0896031675 / ISBN 13: 9780896031678
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Title: Receptor Purification Volume 1 Receptors for...

Publisher: Humana Press

Binding: Hardcover

Book Condition: New

Book Type: Hardcover


Hardcover. 501 pages. Dimensions: 10.1in. x 7.2in. x 1.4in.Receptor Purification is a unique, practical two-volume guide to the purification of most receptor types, including receptors for neurotransmitters, polypeptide hormones, steroid hormones, and a variety of other ligands involved in many biological processes. Todays methods of receptor purification have evolved from years of research on the mechanisms of receptor actions, where they were needed to discover receptor properties and ultimately for use in cloning. Receptor Purification provides a comprehensive source of techniques to enable the purification of cloned receptors, as well as a handy reference on purification of receptors from cells and tissues in a physiological context. Designed to give investigators the best possible selection of approaches to a purification problem, alternative methods are provided where applicable. In addition, Receptor Purification features chapters on specific agents-such as covalent binding ligands-that will greatly simplify some purification processes. Many of the leading laboratories whose work has led to the purification of a wide variety of receptors are represented in the contributions to these two volumes. In some chapters, information has been added on the applications of purified receptors, while others focus on the cloning of the receptors. The purification of receptors is group-based on functional ligands. The first volume is devoted to the purification of receptors that bind neurotransmitters and a wide variety of polypeptide hormones. Volume 2 covers the steroid receptors and associated proteins, such as vitamin D-3 and retinoic acid receptors. Other polypeptide receptors, such as those involved in bacterial motion, are given attention as well. In short, the twin volumes of Receptor Purification constitute the most complete collection of purification procedures to date. Receptor Purification is a valuable desk- and benchside resource for biologists, biochemists, pharmacologists, molecular biologists, endocrinologists, and indeed everyone-whether novice or expert-involved in any area of receptor research. This item ships from multiple locations. Your book may arrive from Roseburg,OR, La Vergne,TN. Bookseller Inventory # 9780896031678

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Synopsis: The purpose of these volumes is to provide a reference work for the methods of purifying many of the receptors we know about. This be­ comes increasingly important as full-length receptors are overexpressed in bacteria or in insect cell systems. A major problem for abundantly expressed proteins will be their purification. In addition to purification protocols, many other details can be found concerning an individual receptor that may not be available in standard texts or monographs. No book of this type is available as a compendium of purification procedures. Receptor Purification provides protocols for the purification of a wide variety of receptors. These include receptors that bind: neurotransmit­ ters, polypeptide hormones, steroid hormones, and ligands for related members of the steroid supergene family and others, including receptors involved in bacterial motion. The text of this information is substantial, so as to require its publication in two volumes. Consequently, a division was made by grouping receptors by the nature of their ligands. Thus, in Volume One there are contributions on serotonin receptors, adrenergic receptors, the purification of GTP-binding proteins, opioid receptors, neurotensin receptor, luteinizing hormone receptor, human chorionic gonadotropin receptor, follicle stimulating hormone receptor, thyro­ tropin receptor, prolactin receptor, epidermal growth factor receptor, platelet derived growth factor receptor, colony stimulating factor recep­ tor, insulin-like growth factor receptors, insulin receptor, fibronectin receptor, interferon receptor, and the cholecystokinin receptor.

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