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Single Molecule Techniques: A Laboratory Manual

Paul R. Selvin

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ISBN 10: 087969775X / ISBN 13: 9780879697754
Published by Cold Spring Harbor Laboratory Press,U.S.
New Condition: New Soft cover
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Bibliographic Details

Title: Single Molecule Techniques: A Laboratory ...

Publisher: Cold Spring Harbor Laboratory Press,U.S.

Binding: Paperback

Book Condition:New

About this title

Synopsis:

As molecular and cellular biologists move toward nanotechniques for performing experiments on single molecules rather than on populations of molecules, a comprehensive manual on how (and why) to carry out such experiments is needed. Single-Molecule Techniques: A Laboratory Manual fills this requirementit is the first to take researchers who know nothing about singlemolecule analyses to the point where they can successfully design and execute appropriate experiments. Geared toward research scientists in structural and molecular biology, biochemistry, and biophysics, the manual will be useful to all who are interested in observing, manipulating, and elucidating the molecular mechanisms and discrete properties of macromolecules. Techniques range from in vivo and in vitro fluorescentbased methods to the use of atomic force microscopy, optical and magnetic tweezers, and nanopores. The book is edited by Paul R. Selvin and Taekjip Ha, two pioneers in the field of experimental biophysics who have made significant contributions to the development and application of singlemolecule technologies

Review:

''I don't believe I have ever before read a microscopy textbook from cover to cover--but this one, reader, I did. Its two editors, Taekjip Ha and Paul Selvin, have set out to assemble a manual for cell biologists who want to extend their technical repertoires to include single-molecule techniques. They have succeeded admirably, and as a bonus the book provides a fascinating insight into where the technical limits lie, of how we stand--as the authors suggest in their introduction--at the beginning of a new era of single-molecule-based cell biology...This excellent book will be invaluable to anyone who is already competent at multimolecule data collection and interested in getting down to molecular mechanisms.'' --Nature Cell Biology

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