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Buchwalow, Igor B.; Böcker, Werner

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ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer. Hardcover. Condition: Good. 3642046088 Item in good condition. Textbooks may not include supplemental items i.e. CDs, access codes etc.EX LIBRARY BOOK. Seller Inventory # Z3642046088Z3

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Igor B. Buchwalow

Published by Springer-Verlag Berlin and Heidelberg GmbH and Co. KG (2010)

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer-Verlag Berlin and Heidelberg GmbH and Co. KG, 2010. HRD. Condition: New. New Book. Delivered from our US warehouse in 10 to 14 business days. THIS BOOK IS PRINTED ON DEMAND.Established seller since 2000. Seller Inventory # IP-9783642046087

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Igor B. Buchwalow

Published by Springer-Verlag Berlin and Heidelberg GmbH and Co. KG (2010)

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer-Verlag Berlin and Heidelberg GmbH and Co. KG, 2010. HRD. Condition: New. New Book.Shipped from US within 10 to 14 business days.THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. Seller Inventory # IP-9783642046087

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IGOR B. BUCHWALOW

Published by Springer (2010)

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer, 2010. Hardback. Condition: NEW. 9783642046087 This listing is a new book, a title currently in-print which we order directly and immediately from the publisher. For all enquiries, please contact Herb Tandree Philosophy Books directly - customer service is our primary goal. Seller Inventory # HTANDREE0347973

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Buchwalow, Igor B.; B?cker, Werner

Published by Springer (2010)

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer, 2010. Hardcover. Condition: New. Seller Inventory # INGM9783642046087

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Buchwalow, Igor B.

Published by Springer (2018)

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer, 2018. Hardcover. Condition: New. Never used! This item is printed on demand. Seller Inventory # 3642046088

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Buchwalow, Igor B.

Published by Springer (2016)

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer, 2016. Paperback. Condition: New. PRINT ON DEMAND Book; New; Publication Year 2016; Not Signed; Fast Shipping from the UK. No. book. Seller Inventory # ria9783642046087_lsuk

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Igor B. Buchwalow, Werner B?cker

Published by Springer (2010)

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer, 2010. Hardcover. Condition: New. 2010. Seller Inventory # DADAX3642046088

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Igor B. Buchwalow; Werner Böcker

Published by Springer (2010)

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer, 2010. Condition: Good. A+ Customer service! Satisfaction Guaranteed! Book is in Used-Good condition. Pages and cover are clean and intact. Used items may not include supplementary materials such as CDs or access codes. May show signs of minor shelf wear and contain limited notes and highlighting. Seller Inventory # 3642046088-2-4

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Igor B. Buchwalow; Werner B?cker

Published by Springer (2010)

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer, 2010. Hardcover. Condition: Used: Good. Seller Inventory # SONG3642046088

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Igor B. Buchwalow; Werner Böcker

Published by Springer (2010)

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer, 2010. Condition: New. This item is printed on demand for shipment within 3 working days. Seller Inventory # LP9783642046087

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Buchwalow, Igor B.; Böcker, Werner

Published by Springer

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer. Hardcover. Condition: New. 3642046088 Special order direct from the distributor. Seller Inventory # ING9783642046087

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Igor B. Buchwalow; Werner Böcker

Published by Springer (2010)

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer, 2010. Hardcover. Condition: New. book. Seller Inventory # M3642046088

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Igor B. Buchwalow, Werner Böcker

Published by Springer-Verlag Berlin and Heidelberg GmbH Co. KG, Germany (2010)

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer-Verlag Berlin and Heidelberg GmbH Co. KG, Germany, 2010. Hardback. Condition: New. 2010 ed.. Language: English . Brand New Book ***** Print on Demand *****.Combiningtwodifferentscienti?cdisciplines-morphologyandim munochemistry- immunohistochemistryhasdevelopedasanimportantinstrumentinresearchand clinicalpathology. A basicunderstandingofunderlying principlesandpotential problemsisunavoidableifyouwanttobesuccessfulinyouruseofimmunohis- chemistry,aswellasingettingyourpaperspublishedandyourresearchgrants funded. Whilemanyexcellenttextsandmonographsexistwhichcovervariousaspects ofimmunohistochemistry,thelackofaconcisecomprehensiveguidetousing thesemethodswasamajormotivationforwritingthisbook. Ourintentionwasto createaneasy-to-readandfocusedresourcebasedonstate-of-the-artinformation forabroadaudiencerangingfromstudentsandtechnicalassistantstoexperienced researchers. Thishandbookhasaconciseformat,withprotocolsandinstructionsfo r methodsimmediatelyfollowingtheshortintroductorytheoreticalmaterialineach chapter. BeingconsciousofthegrowingroleofInternetasaninformationsource, wehavefounditreasonableinmanycasestosubstitutecitingbooksandjournal publications with corresponding Internet websites. Where possible, commercial sourcesofreagents,kits,andequipmentarelistedthroughoutthetextinstea dof inaseparateindex. Thougheachchapterissmallandintroductory,thishandbook itself is self-suf?cient and provides a comprehensive look at the principles of immunohistochemistry. For readers wanting further depth of knowledge, each chapterisbackedupbyashortlistofcarefullyselectedoriginalarticles. Duringthelastdecade,pioneeringeffortsofhistochemistshaveledtoan- menseimprovementinthereagentsandprotocols. Theresearcherisurgedalwaysto determinethereasonforeverymethodandstepbeforedoingit. Thishandbookis intendedtohelpreaderstoavoidtroublesinthechoiceofanadequatemethod,which happenswhenusingstandardtextbooks. Forthishandbook,wecarefullyselected establishedmethodsandeasy-to-adoptprotocols,payingattentiontomoderndev- opmentsinimmunohistochemistry,suchasantigenretrieval,signalampl i?cation, the use of epitope tags in immunohistochemistry, multiple immunolabeling or diagnosticimmunohistochemistry. Eachofthemethodsdescribedinthishandbook v vi Preface was provedby the authors; many of these methods are routinely used in daily practiceintheirinstitute. Allthepracticalmethodsadvocatedareclearlydescribed, withaccompanyingtables,andtheresultsobtainableareillustratedwithcolour micrographs. Acknowledgements We thank Vera Samoilova forthe perfect technical assistance and other colleaguesfromtheMunsterUniversityClinicforsharingprobesandreagents. IgorB. BuchwalowandWernerBocker Munster Contents 1 AntibodiesforImmunohistochemistry .1 1. 1 StructureofAntibodies .2 1. 2 PolyclonalAntibodies .4 1. 3 MouseMonoclonalAntibodies .4 1. 4 RabbitMonoclonalAntibodies .5 1. 5 ProteinAandProteinGinImmunohistochemistry .7 References .8 2 AntibodyLabelingandtheChoiceoftheLabel .9 2. 1 CovalentLabelingofAntibodies .9 2. 2 Non-CovalentLabelingofPrimaryAntibodieswith LabeledFabFragments .10 2. 3 EnzymeLabelsforLightMicroscopy .13 2. 4 FluorophoreLabelsforFluorescenceMicroscopy .15 2. 5 ColloidalGoldLabelsforElectronMicroscopy .16 References .17 3 ProbesProcessinginImmunohistochemistry .19 3. 1 FixationinImmunohistochemistry .19 3. 1. 1 FixationinAlcoholsandAcetone .20 3. 1. 2 FixationinFormaldehyde .20 3. 1. 3 EffectofFormaldehydeFixationon Antigen-nster Contents 1 AntibodiesforImmunohistochemistry .1 1. 1 StructureofAntibodies .2 1. 2 PolyclonalAntibodies .4 1. 3 MouseMonoclonalAntibodies .4 1. 4 RabbitMonoclonalAntibodies .5 1. 5 ProteinAandProteinGinImmunohistochemistry .7 References .8 2 AntibodyLabelingandtheChoiceoftheLabel .9 2. 1 CovalentLabelingofAntibodies .9 2. 2 Non-CovalentLabelingofPrimaryAntibodieswith LabeledFabFragments .10 2. 3 EnzymeLabelsforLightMicroscopy .13 2. 4 FluorophoreLabelsforFluorescenceMicroscopy .15 2. 5 ColloidalGoldLabelsforElectronMicroscopy .16 References .17 3 ProbesProcessinginImmunohistochemistry .19 3. 1 FixationinImmunohistochemistry .19 3. 1. 1 Fixati. Seller Inventory # APC9783642046087

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Igor B. Buchwalow

Published by Springer

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer. Hardcover. Condition: New. 153 pages. Dimensions: 9.4in. x 6.3in. x 0.6in.Combiningtwodifferentscienticdisciplinesmorphologyandimmunochemistry immunohistochemistryhasdevelopedasanimportantinstrumentinresearc hand clinicalpathology. A basicunderstandingofunderlying principlesandpotential problemsisunavoidableifyouwanttobesuccessfulinyouruseofimmunohis- chemistry, aswellasingettingyourpaperspublishedandyourresearchgrants funded. Whilemanyexcellenttextsandmonographsexistwhichcovervariousaspects ofimmunohistochemistry, thelackofaconcisecomprehensiveguidetousing thesemethodswasamajormotivationforwritingthisbook. Ourintentionwasto createaneasy-to-readandfocusedresourcebasedonstate-of-the-artinformation forabroadaudiencerangingfromstudentsandtechnicalassistantstoexperienced researchers. Thishandbookhasaconciseformat, withprotocolsandinstructionsfor methodsimmediatelyfollowingtheshortintroductorytheoreticalmaterialineach chapter. BeingconsciousofthegrowingroleofInternetasaninformationsource, wehavefounditreasonableinmanycasestosubstitutecitingbooksandjournal publications with corresponding Internet websites. Where possible, commercial sourcesofreagents, kits, andequipmentarelistedthroughoutthetextinsteadof inaseparateindex. Thougheachchapterissmallandintroductory, thishandbook itself is self-sufcient and provides a comprehensive look at the principles of immunohistochemistry. For readers wanting further depth of knowledge, each chapterisbackedupbyashortlistofcarefullyselectedoriginalarticles. Duringthelastdecade, pioneeringeffortsofhistochemistshaveledtoan- menseimprovementinthereagentsandprotocols. Theresearcherisurgedalwaysto determinethereasonforeverymethodandstepbeforedoingit. Thishandbookis intendedtohelpreaderstoavoidtroublesinthechoiceofanadequatemethod, which happenswhenusingstandardtextbooks. Forthishandbook, wecarefullyselected establishedmethodsandeasy-to-adoptprotocols, payingattentiontomoderndev- opmentsinimmunohistochemistry, suchasantigenretrieval, signalamplication, the use of epitope tags in immunohistochemistry, multiple immunolabeling or diagnosticimmunohistochemistry. Eachofthemethodsdescribedinthishandbook v vi Preface was provedby the authors; many of these methods are routinely used in daily practiceintheirinstitute. Allthepracticalmethodsadvocatedareclearlydescribed, withaccompanyingtables, andtheresultsobtainableareillustratedwithcolour micrographs. Acknowledgements We thank Vera Samoilova forthe perfect technical assistance and other colleaguesfromtheMunsterUniversityClinicforsharingprobesandreagents. IgorB. BuchwalowandWernerBocker Munster Contents 1 AntibodiesforImmunohistochemistry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1. 1 StructureofAntibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 1. 2 PolyclonalAntibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 1. 3 MouseMonoclonalAntibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 1. 4 RabbitMonoclonalAntibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1. 5 ProteinAandProteinGinImmunohistochemistry . . . . . . . . . . . . . . . . . . . 7 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 2 AntibodyLabelingandtheChoiceoftheLabel . . . . . . . . . . . . . . . . . . . . . . . . 9 2. 1 CovalentLabelingofAntibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 2. 2 Non-CovalentLabelingofPrimaryAntibodieswith LabeledFabFragments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2. 3 EnzymeLabelsforLightMicroscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 2. 4 FluorophoreLabelsforF This item ships from multiple locations. Your book may arrive from Roseburg,OR, La Vergne,TN. Hardcover. Seller Inventory # 9783642046087

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Igor B. Buchwalow

Published by Springer (2010)

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer, 2010. Hardcover. Condition: Good. 2010. Ships with Tracking Number! INTERNATIONAL WORLDWIDE Shipping available. May not contain Access Codes or Supplements. May be ex-library. Shipping & Handling by region. Buy with confidence, excellent customer service!. Seller Inventory # 3642046088

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Buchwalow, Igor B.; Böcker, Werner

Published by Springer

ISBN 10: 3642046088 ISBN 13: 9783642046087

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About this Item: Springer. Hardcover. Condition: Fine. 3642046088 Like New Condition. Seller Inventory # LN7.1769797

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Buchwalow, Igor B.; Böcker, Werner

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About this Item: Springer. Hardcover. Condition: New. 3642046088 New Condition. Seller Inventory # NEW7.1769797

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