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Dubey

Published by S Chand

ISBN 10: 8121926084 ISBN 13: 9788121926089

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From: BookVistas (New Delhi, DELHI, India)

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About this Item: S Chand. Condition: New. Seller Inventory # UDH-9788121926089

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Dr. R.C. Dubey

Published by S. Chand & Company Ltd (2013)

ISBN 10: 8121926084 ISBN 13: 9788121926089

Softcover
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About this Item: S. Chand & Company Ltd, 2013. Softcover. Condition: New. 5th or later edition. CONTENTS: Unit I : INTRODUCTION : 1. Biotechnology : Scope and Importance : What is Biotechnology?; History of Biotechnology; Traditional Biotechnology; Modern Biotechnology - Emergence of Modern Biotechnology, Biotechnology as an Interdisciplinary Area, Global Impact and Current Excitement of Biotechnology (Health Care, Agriculture, Human Genome Project, Environment, Genomics and Proteomics, Bioinformatic); Global Impact of Biotechnology; Health Care; Agriculture; Environment; Biotechnology in India and Global Trends; Global Scenario; Potential of Modern Biotechnology; International Centre for Genetic Engineering and Biotechnology (ICGEB); Need for Future Development; Achievements of Biotechnology; Ban on Genetic Food, Prevention of Misuse of Biotechnology; Biodiversity and its Conservation (Genetic Diversity, Species Diversity, Ecosystem Diversity); Biodiversity in India; Conservation of Biodiversity Gene Bank and Plant Conservation Unit II : GENES AMD GENETIC ENGINEERING : 2. Genes : Nature, Concept and Synthesis : Chemical Nature of DMA; Chemical Composition; Nucleotides, Nucleosides; Polynucleotides; Chargaff`s Rule of Equivalence; Physical Nature of DNA; Watson and Cricks Model of DNA; Circular and Superhelical DNA; Organisation of DNA in Eukaryotes; Structure of RNA; Gene Concept; Units of a Gene; Cistron; Recon; Mutan; Split Genes (introns); RNA Splicing; Ribozyme; Evolution of Split Genes; Overlapping Gene; Gene Organisation; Gene Expression; Gene Regulation; Transcription; The Lac Operon (Structural Gene, Operator Gene, Promoter Gene and Represser Gene); Artificial Synthesis of Genes; Synthesis of a Gene for Yeast Alanine tRNA; Synthesis of a Gene for Bacterial Tyrosine tRNA; Synthesis of a Human Leukocyte Interferon Gene; Gene Synthesis by using mRNA; Gene Machine; The PCR; Amplification of DNA (Melting of Target DNA, Annealing of Primers, Primer Extension); Application of PCR Technology (Diagnosis of Pathogens, Prenatal Diagnosis, DNA Fingerprinting, in Research, Molecular Archaeology, Diagnosis of Plant Pathogens). 3. Tools of Genetic Engineering - I : Basic Requirements : Gel Permeation; Characteristics Desired for Gel Permeation; Application of Gel Permeation; Ion exchange Chromatography (Cation Exchanger, Anions Exchanger); Electrophoresis; Agarose Gel Electrophoresis; Pulse Field Gel Electrophoresis (PFGE); Polyacrylamide Gel Electrophoresis (PAGE); Sodium Dodecyl Polyacrylamide Gel Electrophoresis (SDS-PAGE); 2D Gel Electrophoresis (iso-electric Focusing); Spec Trophotometry, Matrix Assisted Laser Desorption Ionisation (MALDI), Surface-enhanced Laser Desorption Ionisation (SELDI), Elec Trospray Ionisation (ESI); Spectroscopy, colorimetry, UV-Vis Spectrometry, Fluorescent Spectrornetry; Polymerase Chain Reaction (PCR), Working Mechanism of PCR (Denaturation, Annealing, Hybridization, Reverse Transcription-mediated PCR (RT-PCR), Random Amplified Polyrnerase DNA (RAPD marker), arbitrary-primed PCR (AP-PCR), DNA amplification Fingerprinting (DAF), Application of PCR 4. Tools of Genetic Engineering - II : Cutting and Joining of DNA : Exonucleases; Endonucleases; Restriction Endonucleases; (Types I, II and III) Nomenclature; Example of Some Enzymes; SI Nuclease; DNA Ligases; Alkaline Phosphatase; Reverse Transcriptase; DNA Polymerase; T4 Polynucleotide Kinase, Terminal Transferase; Use of Linkers and Adaptors 5. Tools of Genetic Engineering - III : Cloning Vectors : Bacterial Plasmid Vectors; pBR322, pUC Vectors, pACYC184, pUN12l, Agrobacterium-Based Plasmids, Ti-DNA Plasmid (Mechanism of T-DNA Transfer, Tumour Morphology, Gene Transfer, Properties of Ti-and Ri-based Plasmids; Bacteriophage Vectors, Phage ?-insertion vector, replacement vector (EMBL 3, 4 charon3); phage M 1 3, P phage vector; Cosmids; Phagemid vector, Phagemids-pBlue Script ( + /), pBlue Script IIKS; Yeast Plasmid Vector Printed Pages: 616. Seller Inventory # 9813

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R C DUBEY,

Published by S. Chand Publishing

ISBN 10: 8121926084 ISBN 13: 9788121926089

New

Quantity Available: > 20

From: BookVistas (New Delhi, DELHI, India)

Seller Rating: 3-star rating

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About this Item: S. Chand Publishing. Condition: New. Seller Inventory # Schand-9788121926089

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R C Dubey

ISBN 10: 8121926084 ISBN 13: 9788121926089

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From: University Bookstore (DELHI, DELHI, India)

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About this Item: Condition: New. This is Brand NEW. Seller Inventory # S.Chand151217-1716

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R. C. Dubey

Published by S Chand Co Ltd, India (2007)

ISBN 10: 8121926084 ISBN 13: 9788121926089

Softcover
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From: The Book Depository EURO (London, United Kingdom)

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About this Item: S Chand Co Ltd, India, 2007. Paperback. Condition: New. Language: N/A. Brand New Book. Seller Inventory # AMO9788121926089

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