From the Back Cover:
The polymerase chain reaction is the most powerful technique currently used in molecular biology laboratories. From its discovery in the early 80s, PCR has blossomed into a method that enables everything from ready mutation of DNA to speedy analysis of tens of thousands of nucleotide sequences daily.
PCR Applications: Protocols for Functional Genomics examines the latest PCR implementations. It complements the other books in the series, PCR Protocols (1990) and PCR Strategies (1995), and techniques discussed are relevant to the now-occurring bioinformatics and proteomics boom.
Key Features:
* focuses on gene discovery, genomics, and DNA array technology
* covers quantitative PCR techniques, including the use of standards and kinetic analysis
* includes statistical refinement of primer design parameters
* illustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR
About the Author:
Michael A. Innis, Chiron Corporation, Emeryville, California, U.S.A.
David H. Gelfand, Roche Molecular Systems, Alameda, California, U.S.A.
John J. Sninsky, Roche Molecular Systems, Alameda, California, U.S.A.
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