Items related to PCR: A Practical Approach (Practical Approach Series)
Relatively simple to perform, yet extremely diverse in its applications, polymerase chain reaction (PCR) has become an important tool in many areas of biomedical research. It enables scientists to produce large quantities of DNA from minute amounts of starting material. This book provides a general introduction to PCR for researchers new to the area. It covers gene analysis at the level of restriction enzyme polymorphism, point mutation and sequence analysis. Methods for using different source materials - cloned DNA, genomic DNA, RNA, nucleic acid from archive material and PCR products themselves - are given. The emphasis throughout is on the practical applications of the technique and detailed experimental protocols are included at all stages, as well as valuable advice on avoiding pitfalls and optimizing results. Although other manuals exist, this is the most up-to-date work of its kind and has the advantage of being presented in the popular Practical Approach format. It will be of value to researchers and advanced students working in a wide variety of fields, from molecular biology to medicine.
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About the Author:
"This volume should be useful for those who have some experience in the PCR method and wish to extend the technology to more sophisticated and newer applications." --American Journal of Physiology
"The text and the procedures in these chapters and throughout the book are clearly written and often aided by the use of graphics and photographs of key elements. . . . an excellent presentation and organization of procedureal materials related to PCR." --Journal of the American Chemical Society
M.J. McPherson and P. Quirke are both at the University of Leeds. G.R. Taylor is with the Yorkshire Regional DNA Laboratory.
Review:
"This volume should be useful for those who have some experience in the PCR method and wish to extend the technology to more sophisticated and newer applications." --American Journal of Physiology
"The text and the procedures in these chapters and throughout the book are clearly written and often aided by the use of graphics and photographs of key elements. . . . an excellent presentation and organization of procedureal materials related to PCR." --Journal of the American Chemical Society
"About this title" may belong to another edition of this title.
- PublisherIRL Press
- Publication date1991
- ISBN 10 0199631964
- ISBN 13 9780199631964
- BindingPaperback
- Edition number1
- Number of pages280
- EditorMcPherson M. J., Quirke P., Taylor G. R.
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