Gene Transfer and Expression: A Laboratory Manual

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9780716770046: Gene Transfer and Expression: A Laboratory Manual
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This book presents a descriptive, comprehensive catalogue of viral and cellular cis-acting elements and the latest expression vectors for obtaining optimum expression of foreign genes introduced into mammalian cells. Included is a selection of optimized, state-of-the-art protocols for transferring genes into mammalian cells and subsequently assaying for transfer and expression of the genes of interest. This unique volume describes in detail essential techniques for propagating cells and cell lines, DNA transfer techniques, drug selection and gene amplification techniques, expression cloning, subtractive hybridization and retrovirus-mediated gene transfer techniques. Also featured are highly sensitive assays for gene transfer and expression, including detection of DNA and RNA by both isotopic and non-isotopic detection methods as well as the most sensitive non-isotopic Western techniques for the detection of proteins.

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About the Author:

Michael Kriegler is at Cytel Corporation.

Review:


"Set to become a real bench manual that should not remain gathering dust on the bookshelves of all its enthusiastic buyers. . . . many will find in this book a great deal of important information that will increase their confidence and precision during experimentation. First, the aim of the manuals is limited to specific, important techniques. Second, the book is well written: the techniques are clearly presented and up-to-date. . . . well explained and should be clear to the novice. . . . will certainly be useful in many laboratories. The organization of the methods is very logical." --Cell


"Excellent. It is written in a wonderfully direct and clear style. . . . very cost-effective. It deals with an expert area that has not been covered in detail before. The protocols themselves, the heart of any manual, are also clear and direct. . . . the author really knows they will work. . . . very comprehensive and covers the whole area of eukaryotic expression from retroviral vectors to expression cloning." --Nature


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Kriegler, Michael
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ISBN 10: 0716770040 ISBN 13: 9780716770046
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