A-Z of Quantitative PCR (IUL Biotechnology, No. 5) - Hardcover

Bustin, Stephen A.

Hardcover

ISBN 10: 0963681788 ISBN 13: 9780963681782

Publisher: International University Line, 2004

Synopsis

Bustin (School of Medicine and Dentistry, University of London, UK) and contributors explain features of quantitative PCR (qPCR) and provide alternative viewpoints and methods in this hands-on manual for novice and expert researchers who want to learn how to set up and carry out qPCR assays from scratch. Early chapters give insight on factors that influence a PCR experiment and principles underlying real-time quantification. The core of the book details the steps involved in conducting a qPCR experiment, from sample collection to interpretation of results. Final chapters provide a protocol for mRNA quantification and guidelines for standardization of qPCR assays. Annotation ©2004 Book News, Inc., Portland, OR (booknews.com)

About this title

Review:

A book that should be a must read for anyone wanting to get into the field of real-time quantitative PCR -- Gregory L. Shipley, Ph. D., Director, Quantitative Genomics Core Laboratory, Department of Integrative Biology and Pharmacology, The University of Texas Health Science Center-Houston

This book will quickly become an essential reference manual for every lab wanting to perform quantitative PCR -- David G. Ginzinger, Ph. D., Laboratory Director, Genome Analysis Core, UCSF Comprehensive Cancer Center

About this edition

• Publisher: International University Line
• Publication date: 2004
• ISBN 10: 0963681788
• ISBN 13: 9780963681782
• Binding: Hardcover
• Edition number: 1
• Number of pages: 910