Items related to Antitumor Activity of L-Asparaginase from Chicken liver:...
This study aimed to isolate, purify and characterize L-asparaginase present in some animal tissues. The L-asparaginase (EC 3.5.1.1) produced by chicken livers was isolated and characterized. Different purification steps (including ammonium sulphate fractionation followed by separation on Sephadex G-100 gel filtration and Sephadex G-200 gel filtration) were applied to crude filtrate to obtain a pure enzyme preparation. The enzyme was purified 128.5 ± 0.5 fold and showed a final specific activity of 158.11 ± 5.0 U/mg with a 17.1 ± 8.6 % yield. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of the purified enzyme revealed it was one peptide chain with Mr of 33 kDa while by gel filtration appears to be 36 kDa. The enzyme was very specific to L-asparagine and did not hydrolyze L-glutamine. A Lineweaver-Burk analysis showed a Km value of 1.66 mM toward L-asparagine as substrate and Vmax of 34.47 U. The enzyme showed maximum activity at pH 11.0 when incubated at 60◦C for 20 min. The amino acids composition of the purified enzyme was also determined. Antitumor activity was investigated. The enzyme inhibited the growth of the two human cell lines including hepatoc
"synopsis" may belong to another edition of this title.
About the Author:
I have graduated from the faculty of pharmacy with a degree very good with honor I promoted for Prof. of biochemistry (2002) and head of biochemistry department.I have many scientific activities(e.g. Supervised on several thesis,Principle investigator on Research Projects, and Scientific publications of many articles in international journals).
"About this title" may belong to another edition of this title.
- PublisherLAP LAMBERT Academic Publishing
- Publication date2012
- ISBN 10 3659115770
- ISBN 13 9783659115776
- BindingPaperback
- Number of pages116
Buy New
Learn more about this copy
US$ 50.69
Shipping:
US$ 3.99
Within U.S.A.
Top Search Results from the AbeBooks Marketplace
Stock Image
Antitumor Activity of L-Asparaginase from Chicken liver: L-Asparaginase
Published by
LAP LAMBERT Academic Publishing
(2012)
ISBN 10: 3659115770
ISBN 13: 9783659115776
New
Softcover
Quantity: > 20
Seller:
Rating
Book Description Condition: New. Seller Inventory # ABLIING23Mar3113020263313
Buy New
US$ 50.69
Convert currency
Stock Image
Antitumor Activity of L-Asparaginase from Chicken liver
Published by
LAP Lambert Academic Publishing
(2012)
ISBN 10: 3659115770
ISBN 13: 9783659115776
New
Softcover
Quantity: > 20
Seller:
Rating
Book Description Condition: New. PRINT ON DEMAND Book; New; Fast Shipping from the UK. No. book. Seller Inventory # ria9783659115776_lsuk
Buy New
US$ 56.50
Convert currency
Stock Image
Antitumor Activity of LAsparaginase from Chicken liver LAsparaginase
Published by
LAP LAMBERT Academic Publishing
(2012)
ISBN 10: 3659115770
ISBN 13: 9783659115776
New
PAP
Quantity: > 20
Seller:
Rating
Book Description PAP. Condition: New. New Book. Shipped from UK. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. Seller Inventory # L0-9783659115776
Buy New
US$ 76.35
Convert currency
Seller Image
Antitumor Activity of L-Asparaginase from Chicken liver
Published by
LAP Lambert Academic Publishing
(2012)
ISBN 10: 3659115770
ISBN 13: 9783659115776
New
Taschenbuch
Quantity: 2
Seller:
Rating
Book Description Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This study aimed to isolate, purify and characterize L-asparaginase present in some animal tissues. The L-asparaginase (EC 3.5.1.1) produced by chicken livers was isolated and characterized. Different purification steps (including ammonium sulphate fractionation followed by separation on Sephadex G-100 gel filtration and Sephadex G-200 gel filtration) were applied to crude filtrate to obtain a pure enzyme preparation. The enzyme was purified 128.5 ± 0.5 fold and showed a final specific activity of 158.11 ± 5.0 U/mg with a 17.1 ± 8.6 % yield. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of the purified enzyme revealed it was one peptide chain with Mr of 33 kDa while by gel filtration appears to be 36 kDa. The enzyme was very specific to L-asparagine and did not hydrolyze L-glutamine. A Lineweaver-Burk analysis showed a Km value of 1.66 mM toward L-asparagine as substrate and Vmax of 34.47 U. The enzyme showed maximum activity at pH 11.0 when incubated at 60 C for 20 min. The amino acids composition of the purified enzyme was also determined. Antitumor activity was investigated. The enzyme inhibited the growth of the two human cell lines including hepatoc 116 pp. Englisch. Seller Inventory # 9783659115776
Buy New
US$ 54.00
Convert currency
Stock Image
Antitumor Activity of LAsparaginase from Chicken liver LAsparaginase
Published by
LAP LAMBERT Academic Publishing
(2012)
ISBN 10: 3659115770
ISBN 13: 9783659115776
New
PAP
Quantity: > 20
Seller:
Rating
Book Description PAP. Condition: New. New Book. Delivered from our UK warehouse in 4 to 14 business days. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. Seller Inventory # L0-9783659115776
Buy New
US$ 58.58
Convert currency
Seller Image
Antitumor Activity of L-Asparaginase from Chicken liver : L-Asparaginase
Published by
LAP Lambert Academic Publishing
(2012)
ISBN 10: 3659115770
ISBN 13: 9783659115776
New
Taschenbuch
Quantity: 2
Seller:
Rating
Book Description Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - This study aimed to isolate, purify and characterize L-asparaginase present in some animal tissues. The L-asparaginase (EC 3.5.1.1) produced by chicken livers was isolated and characterized. Different purification steps (including ammonium sulphate fractionation followed by separation on Sephadex G-100 gel filtration and Sephadex G-200 gel filtration) were applied to crude filtrate to obtain a pure enzyme preparation. The enzyme was purified 128.5 ± 0.5 fold and showed a final specific activity of 158.11 ± 5.0 U/mg with a 17.1 ± 8.6 % yield. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of the purified enzyme revealed it was one peptide chain with Mr of 33 kDa while by gel filtration appears to be 36 kDa. The enzyme was very specific to L-asparagine and did not hydrolyze L-glutamine. A Lineweaver-Burk analysis showed a Km value of 1.66 mM toward L-asparagine as substrate and Vmax of 34.47 U. The enzyme showed maximum activity at pH 11.0 when incubated at 60 C for 20 min. The amino acids composition of the purified enzyme was also determined. Antitumor activity was investigated. The enzyme inhibited the growth of the two human cell lines including hepatoc. Seller Inventory # 9783659115776
Buy New
US$ 55.56
Convert currency
Seller Image
Antitumor Activity of L-Asparaginase from Chicken liver
Published by
LAP LAMBERT Academic Publishing
(2012)
ISBN 10: 3659115770
ISBN 13: 9783659115776
New
Softcover
Quantity: > 20
Seller:
Rating
Book Description Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: El-Sayed SanaaI have graduated from the faculty of pharmacy with a degree very good with honor I promoted for Prof. of biochemistry (2002) and head of biochemistry department.I have many scientific activities(e.g. Supervised on sever. Seller Inventory # 5132429
Buy New
US$ 45.92
Convert currency