The trypanocidal effects of antibodies to T.brucei-recombinant tubulin: An Evaluation of in Vitro Trypanocidal Activity of Antibodies to Recombinant Tubulin from Trypanosoma B. Brucei

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The trypanocidal effects of antibodies to T.brucei-recombinant tubulin: An Evaluation of in Vitro Trypanocidal Activity of Antibodies to Recombinant Tubulin from Trypanosoma B. Brucei. Bookseller Inventory #

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Synopsis: T. b. brucei alpha and beta tubulin genes were expressed in E. coli using the pTrP2 expression plasmid and 3-β-Indoleacrylic acid as an inducer. The expressed tubulins were analysed by SDS-PAGE and confirmed by Western blotting, in which they had an identical apparent molecular mass of 50kDa. The expressed tubulins were individually dissolved in 8M Urea and diluted 20-fold, after which they were concentrated and extensively dialyzed against MES buffer. Following dialysis, the average yield for both alpha and beta tubulin was 0.7g per liter of culture. Polyclonal antibodies were produced in each of the 56 rabbits immunized with three doses of the concentrated tubulins at either 150μg or 450μg per immunization per animal. The effect of these antibodies on T. b. brucei growth was studied by culturing T. b. brucei bloodstream form in up to 25% of the rabbit-raised antisera. The antisera from the other rabbits did not inhibit T. b. brucei growth in culture. These results show that antibodies to bacterially expressed trypanosome tubulin may not be cytotoxic to T. b. brucei.

About the Author: David Kateete (PhD student, Makerere University, Kampala, Uganda)is currently in his final year of training. He is interested inpathogen biology and currently is studying Cell-cell Signalingin bacteria. He is also a Lecturer in the department of MedicalMicrobiology, Makerere University College of Health Sciences.

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Book Description LAP Lambert Academic Publishing Feb 2013, 2013. Taschenbuch. Book Condition: Neu. Neuware - T. b. brucei alpha and beta tubulin genes were expressed in E. coli using the pTrP2 expression plasmid and 3- -Indoleacrylic acid as an inducer. The expressed tubulins were analysed by SDS-PAGE and confirmed by Western blotting, in which they had an identical apparent molecular mass of 50kDa. The expressed tubulins were individually dissolved in 8M Urea and diluted 20-fold, after which they were concentrated and extensively dialyzed against MES buffer. Following dialysis, the average yield for both alpha and beta tubulin was 0.7g per liter of culture. Polyclonal antibodies were produced in each of the 56 rabbits immunized with three doses of the concentrated tubulins at either 150 g or 450 g per immunization per animal. The effect of these antibodies on T. b. brucei growth was studied by culturing T. b. brucei bloodstream form in up to 25% of the rabbit-raised antisera. The antisera from the other rabbits did not inhibit T. b. brucei growth in culture. These results show that antibodies to bacterially expressed trypanosome tubulin may not be cytotoxic to T. b. brucei. 92 pp. Englisch. Bookseller Inventory # 9783838351407

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Book Description LAP Lambert Academic Publishing Feb 2013, 2013. Taschenbuch. Book Condition: Neu. Neuware - T. b. brucei alpha and beta tubulin genes were expressed in E. coli using the pTrP2 expression plasmid and 3- -Indoleacrylic acid as an inducer. The expressed tubulins were analysed by SDS-PAGE and confirmed by Western blotting, in which they had an identical apparent molecular mass of 50kDa. The expressed tubulins were individually dissolved in 8M Urea and diluted 20-fold, after which they were concentrated and extensively dialyzed against MES buffer. Following dialysis, the average yield for both alpha and beta tubulin was 0.7g per liter of culture. Polyclonal antibodies were produced in each of the 56 rabbits immunized with three doses of the concentrated tubulins at either 150 g or 450 g per immunization per animal. The effect of these antibodies on T. b. brucei growth was studied by culturing T. b. brucei bloodstream form in up to 25% of the rabbit-raised antisera. The antisera from the other rabbits did not inhibit T. b. brucei growth in culture. These results show that antibodies to bacterially expressed trypanosome tubulin may not be cytotoxic to T. b. brucei. 92 pp. Englisch. Bookseller Inventory # 9783838351407

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Book Description LAP Lambert Academic Publishing Feb 2013, 2013. Taschenbuch. Book Condition: Neu. This item is printed on demand - Print on Demand Neuware - T. b. brucei alpha and beta tubulin genes were expressed in E. coli using the pTrP2 expression plasmid and 3- -Indoleacrylic acid as an inducer. The expressed tubulins were analysed by SDS-PAGE and confirmed by Western blotting, in which they had an identical apparent molecular mass of 50kDa. The expressed tubulins were individually dissolved in 8M Urea and diluted 20-fold, after which they were concentrated and extensively dialyzed against MES buffer. Following dialysis, the average yield for both alpha and beta tubulin was 0.7g per liter of culture. Polyclonal antibodies were produced in each of the 56 rabbits immunized with three doses of the concentrated tubulins at either 150 g or 450 g per immunization per animal. The effect of these antibodies on T. b. brucei growth was studied by culturing T. b. brucei bloodstream form in up to 25% of the rabbit-raised antisera. The antisera from the other rabbits did not inhibit T. b. brucei growth in culture. These results show that antibodies to bacterially expressed trypanosome tubulin may not be cytotoxic to T. b. brucei. 92 pp. Englisch. Bookseller Inventory # 9783838351407

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Book Description Book Condition: New. Publisher/Verlag: LAP Lambert Academic Publishing | An Evaluation of in Vitro Trypanocidal Activity of Antibodies to Recombinant Tubulin from Trypanosoma B. Brucei | T. b. brucei alpha and beta tubulin genes were expressed in E. coli using the pTrP2 expression plasmid and 3- -Indoleacrylic acid as an inducer. The expressed tubulins were analysed by SDS-PAGE and confirmed by Western blotting, in which they had an identical apparent molecular mass of 50kDa. The expressed tubulins were individually dissolved in 8M Urea and diluted 20-fold, after which they were concentrated and extensively dialyzed against MES buffer. Following dialysis, the average yield for both alpha and beta tubulin was 0.7g per liter of culture. Polyclonal antibodies were produced in each of the 56 rabbits immunized with three doses of the concentrated tubulins at either 150 g or 450 g per immunization per animal. The effect of these antibodies on T. b. brucei growth was studied by culturing T. b. brucei bloodstream form in up to 25% of the rabbit-raised antisera. The antisera from the other rabbits did not inhibit T. b. brucei growth in culture. These results show that antibodies to bacterially expressed trypanosome tubulin may not be cytotoxic to T. b. brucei. | Format: Paperback | Language/Sprache: english | 92 pp. Bookseller Inventory # K9783838351407

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Book Description LAP Lambert Academic Publishing, Germany, 2010. Paperback. Book Condition: New. Language: English . Brand New Book. T. b. brucei alpha and beta tubulin genes were expressed in E. coli using the pTrP2 expression plasmid and 3- -Indoleacrylic acid as an inducer. The expressed tubulins were analysed by SDS-PAGE and confirmed by Western blotting, in which they had an identical apparent molecular mass of 50kDa. The expressed tubulins were individually dissolved in 8M Urea and diluted 20-fold, after which they were concentrated and extensively dialyzed against MES buffer. Following dialysis, the average yield for both alpha and beta tubulin was 0.7g per liter of culture. Polyclonal antibodies were produced in each of the 56 rabbits immunized with three doses of the concentrated tubulins at either 150 g or 450 g per immunization per animal. The effect of these antibodies on T. b. brucei growth was studied by culturing T. b. brucei bloodstream form in up to 25 of the rabbit-raised antisera. The antisera from the other rabbits did not inhibit T. b. brucei growth in culture. These results show that antibodies to bacterially expressed trypanosome tubulin may not be cytotoxic to T. b. brucei. Bookseller Inventory # KNV9783838351407

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