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Book Description Condition: New. Seller Inventory # ABLIING23Mar3113020266095
Book Description Condition: New. PRINT ON DEMAND Book; New; Fast Shipping from the UK. No. book. Seller Inventory # ria9783659147913_lsuk
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Book Description Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Fasciolosis is a liver disease caused by Fasciola hepatica or F. gigantica that causes significant economic loss in cattles, other animal species and man. The appearance of Fasciola hepatica populations that are resistant to common flukicidal drugs make a need for the development of anti-liver fluke vaccines. Though we selected a target Fasciola gene that expresses (rFhp) protein, in which its native form shows significant induction to lymphoproliferative response of Peripheral blood mononuclear cells. Then we amplified the gene through Polymerase chain reaction process using gt11 specific primers. This gene was cloned through TOPO (TA) cloning vector in XL10-Gold competent cells, then positive colonies that contain rTOPO were used for the excision of the gene to be expressed in expression vectors. ECORI digestions of the purified plasmids were done to detect the existence of the target insert. The results showed the excision of Fh 400 from recombinant PQE32 vector and its size determined at 500bp. Expression and screening of small cultures was done and a 6xHis-tagged protein was purified & stained on SDS-PAGE that appeared at about 14.5 kDa. 120 pp. Englisch. Seller Inventory # 9783659147913
Book Description Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Fasciolosis is a liver disease caused by Fasciola hepatica or F. gigantica that causes significant economic loss in cattles, other animal species and man. The appearance of Fasciola hepatica populations that are resistant to common flukicidal drugs make a need for the development of anti-liver fluke vaccines. Though we selected a target Fasciola gene that expresses (rFhp) protein, in which its native form shows significant induction to lymphoproliferative response of Peripheral blood mononuclear cells. Then we amplified the gene through Polymerase chain reaction process using gt11 specific primers. This gene was cloned through TOPO (TA) cloning vector in XL10-Gold competent cells, then positive colonies that contain rTOPO were used for the excision of the gene to be expressed in expression vectors. ECORI digestions of the purified plasmids were done to detect the existence of the target insert. The results showed the excision of Fh 400 from recombinant PQE32 vector and its size determined at 500bp. Expression and screening of small cultures was done and a 6xHis-tagged protein was purified & stained on SDS-PAGE that appeared at about 14.5 kDa. Seller Inventory # 9783659147913
Book Description PAP. Condition: New. New Book. Delivered from our UK warehouse in 4 to 14 business days. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. Seller Inventory # L0-9783659147913
Book Description Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Hussien NahedDr. Nahed Ahmed Hussien, I am a Lecturer at Cairo Univ., Faculty of science, Zoology ?Department from 2010. Contributed at the Egyptian Reference Diagnostic ?Center in Fasciola Vaccine projects, and also in Welcome Trus. Seller Inventory # 5134962