Experiments in Biochemistry: A Hands-On Approach

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9780030212840: Experiments in Biochemistry: A Hands-On Approach

The experiments have been classroom rested through multiple semesters. They are proven to work and can be completed in a normal lab period. Alternate versions of experiments allow for easy use in labs which meet once a week or multiple times a week. The manual also makes it easy for students to use due to six "Tip" boxes located throughout the text, which give pointers on how to perform the labs and six "Essential Information" boxes that highlight pertinent information. There are also references and further reading sections located at the end of each chapter.

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Shawn O. Farrell, a native of Northern California, received his B.S. in biochemistry from University of California, Davis, studying carbohydrate metabolism. He completed his Ph.D. in biochemistry at Michigan State University, where he focused on the study of fatty acid metabolism. Dr. Farrell became interested in biochemistry while in college, as it was relevant to his passion for bicycle racing. He raced competitively for 15 years and now officiates bicycle races worldwide. He has taught biochemistry lecture and laboratory courses at Colorado State University for 16 years and now works for USCycling. Professor Farrell has written scientific journal articles about specific research projects and about laboratory teaching, as well as articles for sports publications, such as "Salmon, Trout, and Steelheader" magazine. He is co-author with Mary Campbell on BIOCHEMISTRY, 7e (Cengage Learning).

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Introduction to the Text. Objectives of the Biochemistry Laboratory. Chapter Format of Hands on Biochemistry. 1. Biochemistry Boot Camp. 2. Acids, Bases, and Buffers. 3. Spectrophotometry. 4. Enzyme Purification. 5. Ion Exchange Chromatography. 6. Affinity Chromatography. 7. Gel filtration Chromatography. 8. Enzyme Kinetics. 9. Electrophoresis. 10. Western Blots. 11. Restriction Enzymes. 12. Cloning and Expression of Foreign Proteins. 13. Polymerase Chain Reaction.

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